Human IL-6R alpha PE-conjugated Antibody Summary
Leu20-Asp339
Accession # P08887
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL-6R alpha in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A) and either (A) Mouse Anti-Human IL-6R alpha PE-conjugated Monoclonal Antibody (Catalog # FAB227P) or (B) Mouse IgG1Phycoerythrin Isotype Control (IC002P). View our protocol for Staining Membrane-associated Proteins.
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Detection of IL-6R alpha in U937 cells by Flow Cytometry U937 cells were stained with Mouse Anti-Human IL-6R alpha PE‑conjugated Monoclonal Antibody (Catalog # FAB227P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-6R alpha
The multi-functional factor Interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).
A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).
- Yamasaki, K. et al. (1988) Science 241:825.
- Baumann, M. et al. (1990) J. Biol. Chem. 265:19853.
- Hibi, M. et al. (1990) Cell 63:1149.
- Schooltink, H. et al. (1991) Eur. J. Biochem. 277:659.
- Novick, D. et al. (1989) J. Exp. Med. 170:1409.
- Yasukawa, K. et al. (1990) J. Biochem. 108:673.
- Saito, T. et al. (1991) J. Immunology 147:168.
Product Datasheets
Citation for Human IL-6R alpha PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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IL-27 driven upregulation of surface HLA-E expression on monocytes inhibits IFN-gamma release by autologous NK cells.
Authors: Morandi F, Airoldi I, Pistoia V
J Immunol Res, 2014-03-10;2014(0):938561.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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