Human IL-7 Quantikine HS ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 0.5 | 1.49 | 6.86 | 0.78 | 2.13 | 8.81 |
Standard Deviation | 0.04 | 0.14 | 0.61 | 0.08 | 0.16 | 0.64 |
CV% | 8 | 9.4 | 8.9 | 10.3 | 7.5 | 7.3 |
Serum, EDTA Plasma, Citrate Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 0.46 | 5.79 | 11.18 | 0.51 | 6.38 | 12.95 |
Standard Deviation | 0.03 | 0.19 | 0.44 | 0.06 | 0.5 | 1.3 |
CV% | 6.5 | 3.3 | 3.9 | 11.8 | 7.8 | 10 |
Recovery
The recovery of IL-7 spiked to three different levels throughout the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=5) | 103 | 89-113 |
Citrate Plasma (n=5) | 100 | 92-110 |
EDTA Plasma (n=5) | 112 | 83-132 |
Serum (n=5) | 100 | 91-113 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: IL-7
IL-7 (Interleukin-7) is a cytokine that plays important roles in lymphocyte differentiation, proliferation, and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestines. It signals through a receptor complex composed of IL-7 R alpha/CD127 and the Common gamma Chain. gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. IL-7 contributes to the maintenance of all naïve and memory T cells. It is required for optimal T cell-dendritic cell interaction. In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 200 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 14-20 hours.
- Aspirate each well and wash, repeating the process 5 times for a total of 6 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 6 times.
- Add 50 µL Substrate Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 45 minutes. Do not wash the plate.
- Add 50 µL Amplifier Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 45 minutes.
- Add 50 µL of Stop Solution to each well. Read at 490 nm within 30 minutes. Set wavelength correction to 650 nm or 690 nm.
Citations for Human IL-7 Quantikine HS ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies
Authors: Chen, S;Nguyen, TD;Lee, KZ;Liu, D;
Biotechnology advances
Species: Human
Sample Types: Cell Culture Supernates
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Roles of IL-7R Induced by Interactions between Cancer Cells and Macrophages in the Progression of Esophageal Squamous Cell Carcinoma
Authors: Y Kitamura, YI Koma, K Tanigawa, S Tsukamoto, Y Azumi, S Miyako, S Urakami, T Kodama, M Nishio, M Shigeoka, Y Kakeji, H Yokozaki
Cancers, 2023-01-06;15(2):.
Species: Human
Sample Types: Cell Culture Supernates
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Association between anti-Anisakis simplex antibodies and interleukin-7 levels
Authors: C Cuéllar, M Rodero, J Pérez-Grie, L Galindo-Re, F Lopez-Chul, C García-Bal, J Carlos And
International immunopharmacology, 2022-08-11;111(0):109134.
Species: Human
Sample Types: Serum
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Targeting the Gut Microbiota of Vertically HIV-Infected Children to Decrease Inflammation and Immunoactivation: A Pilot Clinical Trial
Authors: T Sainz, L Diaz, D Rojo, MI Clemente, C Barbas, MJ Gosalbes, N Jimenez-He, L Escosa, S Guillen, JT Ramos, MÁ Muñoz-Fern, ML Navarro, MJ Mellado, S Serrano-Vi
Nutrients, 2022-02-26;14(5):.
Species: Human
Sample Types: Plasma
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Relationship between CD4 T cell turnover, cellular differentiation and HIV persistence during ART
Authors: C Bacchus-So, M Fitch, J Symons, M Abdel-Mohs, DB Reeves, R Hoh, M Stone, J Hiatt, P Kim, A Chopra, H Ahn, VA York, DL Cameron, FM Hecht, JN Martin, SA Yukl, S Mallal, PU Cameron, SG Deeks, JT Schiffer, SR Lewin, MK Hellerstei, JM McCune, PW Hunt
PloS Pathogens, 2021-01-19;17(1):e1009214.
Species: Human
Sample Types: Plasma
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Mycobacterium tuberculosis co-infection is associated with increased surrogate marker of the HIV reservoir
Authors: J Xun, T Qi, L Zou, Q Tang, Y Shen, J Yang, L Xie, Y Ji, R Zhang, L Liu, J Wang, C Steinhart, Z Wang, Y Tang, W Song, J Sun, J Cheng, X Le, H Wu, X He, R Chen, J Chen, H Lu
AIDS Res Ther, 2020-10-19;17(1):63.
Species: Human
Sample Types: Plasma
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HIV-1 Tat protein inhibits the hematopoietic support function of human bone marrow mesenchymal stem cells
Authors: Y Yuan, C Zhou, Q Yang, S Ma, X Wang, X Guo, Y Ding, J Tang, Y Zeng, D Li
Virus Res., 2019-09-12;273(0):197756.
Species: Human
Sample Types: Cell Culture Supernates
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Altered levels of memory T cell subsets and common ?c cytokines in Strongyloides stercoralis infection and partial reversal following anthelmintic treatment
Authors: A Rajamanick, S Munisankar, Y Bhootra, CK Dolla, K Thiruvenga, TB Nutman, S Babu
PLoS Negl Trop Dis, 2018-05-24;12(5):e0006481.
Species: Human
Sample Types: Plasma
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IL7RA haplotypes differentially affect soluble IL-7 receptor and IL-7 serum concentrations in children with type 1 diabetes
Authors: J Seyfarth, C Lundtoft, K Förtsch, H Ahlert, J Rosenbauer, C Baechle, M Roden, RW Holl, E Mayatepek, S Kummer, T Meissner, M Jacobsen
Pediatr Diabetes, 2018-03-13;0(0):.
Species: Human
Sample Types: Serum
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Aberrant plasma IL-7 and soluble IL-7 receptor levels indicate impaired T-cell response to IL-7 in human tuberculosis
Authors: C Lundtoft, A Afum-Adjei, J Rimpler, K Harling, N Nausch, M Kohns, E Adankwah, F Lang, L Olbrich, E Mayatepek, E Owusu-Dabo, M Jacobsen
PLoS Pathog., 2017-06-05;13(6):e1006425.
Species: Human
Sample Types: Plasma
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Acute Simian Immunodeficiency Virus Infection Triggers Early and Transient Interleukin-7 Production in the Gut, Leading to Enhanced Local Chemokine Expression and Intestinal Immune Cell Homing
Authors: R Ponte, M Rancez, S Figueiredo, J Dutrieux, V Fabre-Mers, B Charmeteau, T Guilbert, JP Routy, R Cheynier, A Couëdel-Co
Front Immunol, 2017-05-19;8(0):588.
Species: Primate
Sample Types: Tissue Homogenates
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Diminished plasma levels of common ?-chain cytokines in pulmonary tuberculosis and reversal following treatment
Authors: NP Kumar, VV Banurekha, D Nair, S Babu
PLoS ONE, 2017-04-27;12(4):e0176495.
Species: Human
Sample Types: Plasma
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Human Lymph Node-Derived Fibroblastic and Double-Negative Reticular Cells Alter Their Chemokines and Cytokines Expression Profile Following Inflammatory Stimuli
Authors: P Severino, DT Palomino, H Alvarenga, CB Almeida, DC Pasqualim, A Cury, PR Salvalaggi, AL De Vasconc, MC Andrade, T Aloia, S Bromberg, LV Rizzo, FA Rocha, LC Marti
Front Immunol, 2017-02-14;8(0):141.
Species: Human
Sample Types: Cell Culture Supernates
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Establishment of IL-7 Expression Reporter Human Cell Lines, and Their Feasibility for High-Throughput Screening of IL-7-Upregulating Chemicals
PLoS ONE, 2016-09-02;11(9):e0161899.
Species: Human
Sample Types: Cell Culture Supernates
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Effect of IL-7 Therapy on Naive and Memory T Cell Homeostasis in Aged Rhesus Macaques.
Authors: Okoye A, Rohankhedkar M, Konfe A, Abana C, Reyes M, Clock J, Duell D, Sylwester A, Sammader P, Legasse A, Park B, Axthelm M, Nikolich-Zugich J, Picker L
J Immunol, 2015-09-28;195(9):4292-305.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Plasma
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Recycled IL-7 Can Be Delivered to Neighboring T Cells.
Authors: Bazdar D, Kalinowska M, Panigrahi S, Sieg S
J Immunol, 2015-04-13;194(10):4698-704.
Species: Human
Sample Types: Cell Culture Supernates
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Immune Recovery after Allogeneic Hematopoietic Stem Cell Transplantation Following Flu-TBI versus TLI-ATG Conditioning.
Authors: Hannon M, Beguin Y, Ehx G, Servais S, Seidel L, Graux C, Maertens J, Kerre T, Daulne C, De Bock M, Fillet M, Ory A, Willems E, Gothot A, Humblet-Baron S, Baron F
Clin Cancer Res, 2015-03-16;21(14):3131-9.
Species: Human
Sample Types: Plasma
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Long-term control of simian immunodeficiency virus (SIV) in cynomolgus macaques not associated with efficient SIV-specific CD8+ T-cell responses.
Authors: Bruel T, Hamimi C, Dereuddre-Bosquet N, Cosma A, Shin S, Corneau A, Versmisse P, Karlsson I, Malleret B, Targat B, Barre-Sinoussi F, Le Grand R, Pancino G, Saez-Cirion A, Vaslin B
J Virol, 2015-01-14;89(7):3542-56.
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Types: Plasma
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T cell reconstitution in allogeneic haematopoietic stem cell transplantation: prognostic significance of plasma interleukin-7.
Authors: Kielsen K, Jordan K, Uhlving H, Pontoppidan P, Shamim Z, Ifversen M, Heilmann C, Nielsen C, Sengelov H, Ryder L, Muller K
Scand J Immunol, 2015-01-01;81(1):72-80.
Species: Human
Sample Types: Plasma
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IFN-alpha exerts opposing effects on activation-induced and IL-7-induced proliferation of T cells that may impair homeostatic maintenance of CD4+ T cell numbers in treated HIV infection.
Authors: Cha L, de Jong E, French M, Fernandez S
J Immunol, 2014-07-25;193(5):2178-86.
Species: Human
Sample Types: Cell Culture Supernates
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Chronic exposure to type-I IFN under lymphopenic conditions alters CD4 T cell homeostasis.
Authors: Le Saout C, Hasley R, Imamichi H, Tcheung L, Hu Z, Luckey M, Park J, Durum S, Smith M, Rupert A, Sneller M, Lane H, Catalfamo M
PLoS Pathog, 2014-03-06;10(3):e1003976.
Species: Human
Sample Types: Serum
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Co-culture of human bronchial fibroblasts and CD4+ T cells increases Th17 cytokine signature.
Authors: Loubaki L, Hadj-Salem I, Fakhfakh R, Jacques E, Plante S, Boisvert M, Aoudjit F, Chakir J
PLoS ONE, 2013-12-05;8(12):e81983.
Species: Human
Sample Types: Cell Culture Supernates
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The systemic cytokine environment is permanently altered in multiple myeloma.
Authors: Zheng M, Zhang Z, Bemis K, Belch A, Pilarski L, Shively J, Kirshner J
PLoS ONE, 2013-03-27;8(3):e58504.
Species: Human
Sample Types: Plasma
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Up-regulation of intestinal epithelial cell derived IL-7 expression by keratinocyte growth factor through STAT1/IRF-1, IRF-2 pathway.
Authors: Cai, Yu-Jiao, Wang, Wen-Shen, Yang, Yang, Sun, Li-Hua, Teitelbaum, Daniel H, Yang, Hua
PLoS ONE, 2013-03-12;8(3):e58647.
Species: Human
Sample Types: Cell Culture Supernates
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Decreased systemic IL-7 and soluble IL-7Ralpha in multiple sclerosis patients.
Genes Immun, 2012-08-23;13(7):587-92.
Species: Human
Sample Types: Plasma
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Treatment with IL-7 prevents the decline of circulating CD4+ T cells during the acute phase of SIV infection in rhesus macaques.
Authors: Vassena L, Miao H, Cimbro R, Malnati MS, Cassina G, Proschan MA, Hirsch VM, Lafont BA, Morre M, Fauci AS, Lusso P
PLoS Pathog., 2012-04-12;8(4):e1002636.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Plasma
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Altered T cell memory and effector cell development in chronic lymphatic filarial infection that is independent of persistent parasite antigen.
Authors: Steel C, Nutman TB
PLoS ONE, 2011-04-29;6(4):e19197.
Species: Human
Sample Types: Serum
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Plasma levels of IL-7 and IL-15 after reduced intensity conditioned allo-SCT and relationship to acute GVHD.
Authors: Thiant S, Labalette M, Trauet J, Coiteux V, de Berranger E, Dessaint JP, Yakoub-Agha I
Bone Marrow Transplant., 2010-12-06;46(10):1374-81.
Species: Human
Sample Types: Plasma
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Lack of anti-tumour reactivity despite enhanced numbers of circulating natural killer T cells in two patients with metastatic renal cell carcinoma.
Authors: Vyth-Dreese FA, Sein J, van de Kasteele W, Dellemijn TA, van den Bogaard C, Nooijen WJ, de Gast GC, Haanen JB, Bex A
Clin. Exp. Immunol., 2010-10-05;162(3):447-59.
Species: Human
Sample Types: Plasma
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Elevated frequencies of highly activated CD4+ T cells in HIV+ patients developing immune reconstitution inflammatory syndrome.
Authors: Antonelli LR, Mahnke Y, Hodge JN, Porter BO, Barber DL, DerSimonian R, Greenwald JH, Roby G, Mican J, Sher A, Roederer M, Sereti I
Blood, 2010-07-26;116(19):3818-27.
Species: Human
Sample Types: Serum
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Peripheral blood B cell subset skewing is associated with altered cell cycling and intrinsic resistance to apoptosis and reflects a state of immune activation in chronic hepatitis C virus infection.
Authors: Sugalski JM, Rodriguez B, Moir S
J. Immunol., 2010-07-23;185(5):3019-27.
Species: Human
Sample Types: Serum
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Plasma levels of IL-7 and IL-15 in the first month after myeloablative BMT are predictive biomarkers of both acute GVHD and relapse.
Authors: Thiant S, Yakoub-Agha I, Magro L, Trauet J, Coiteux V, Jouet JP, Dessaint JP, Labalette M
Bone Marrow Transplant., 2010-03-01;45(10):1546-52.
Species: Human
Sample Types: Plasma
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Dual mechanism of impairment of interleukin-7 (IL-7) responses in human immunodeficiency virus infection: decreased IL-7 binding and abnormal activation of the JAK/STAT5 pathway.
Authors: Juffroy O, Bugault F, Lambotte O, Landires I, Viard JP, Niel L, Fontanet A, Delfraissy JF, Theze J, Chakrabarti LA
J. Virol., 2010-01-01;84(1):96-108.
Species: Human
Sample Types: Plasma
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Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma.
Authors: Cattaruzza L, Gloghini A, Olivo K, Di Francia R, Lorenzon D, De Filippi R, Carbone A, Colombatti A, Pinto A, Aldinucci D
Int. J. Cancer, 2009-09-01;125(5):1092-101.
Species: Human
Sample Types: Cell Culture Supernates
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Identification and biochemical characterization of human plasma soluble IL-7R: lower concentrations in HIV-1-infected patients.
Authors: Rose T, Lambotte O, Pallier C, Delfraissy JF, Colle JH
J. Immunol., 2009-06-15;182(12):7389-97.
Species: Human
Sample Types: Plasma
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Identification of interleukin-7 as a candidate disease mediator in spondylarthritis.
Authors: Rihl M, Kellner H, Kellner W, Barthel C, Yu DT, Tak PP, Zeidler H, Baeten D
Arthritis Rheum., 2008-11-01;58(11):3430-5.
Species: Human
Sample Types: Synovial Fluid
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Enhancing the in vivo expansion of adoptively transferred EBV-specific CTL with lymphodepleting CD45 monoclonal antibodies in NPC patients.
Authors: Louis CU, Straathof K, Bollard CM, Gerken C, Huls MH, Gresik MV, Wu MF, Weiss HL, Gee AP, Brenner MK, Rooney CM, Heslop HE, Gottschalk S
Blood, 2008-10-29;113(11):2442-50.
Species: Human
Sample Types: Plasma
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Longitudinal analysis of immune function in the first 3 years of life in thymectomized neonates during cardiac surgery.
Authors: Mancebo E, Clemente J, Sanchez J, Ruiz-Contreras J, De Pablos P, Cortezon S, Romo E, Paz-Artal E, Allende LM
Clin. Exp. Immunol., 2008-09-22;154(3):375-83.
Species: Human
Sample Types: Plasma
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Sleep enhances serum interleukin-7 concentrations in humans.
Authors: Benedict C, Dimitrov S, Marshall L, Born J
Brain Behav. Immun., 2007-05-23;21(8):1058-62.
Species: Human
Sample Types: Serum
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Lymphocytes and synovial fluid fibroblasts support osteoclastogenesis through RANKL, TNFalpha, and IL-7 in an in vitro model derived from human psoriatic arthritis.
Authors: Colucci S, Brunetti G, Cantatore FP, Oranger A, Mori G, Quarta L, Cirulli N, Mancini L, Corrado A, Grassi FR, Grano M
J. Pathol., 2007-05-01;212(1):47-55.
Species: Human
Sample Types: Serum
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Potential role for IL-7 in Fas-mediated T cell apoptosis during HIV infection.
Authors: Fluur C, De Milito A, Fry TJ, Vivar N, Eidsmo L, Atlas A, Federici C, Matarrese P, Logozzi M, Rajnavolgyi E, Mackall CL, Fais S, Chiodi F, Rethi B
J. Immunol., 2007-04-15;178(8):5340-50.
Species: Human
Sample Types: Serum
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IL-7Ralpha expression on CD4+ T lymphocytes decreases with HIV disease progression and inversely correlates with immune activation.
Authors: Koesters SA, Alimonti JB, Wachihi C, Matu L, Anzala O, Kimani J, Embree JE, Plummer FA, Fowke KR
Eur. J. Immunol., 2006-02-01;36(2):336-44.
Species: Human
Sample Types: Plasma
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Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells.
Authors: Johnson SE, Shah N, Panoskaltsis-Mortari A, Lebien TW
J. Immunol., 2005-12-01;175(11):7325-31.
Species: Human
Sample Types: Cell Culture Supernates
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Elevated interleukin-7 levels not sufficient to maintain T-cell homeostasis during simian immunodeficiency virus-induced disease progression.
Authors: Muthukumar A, Wozniakowski A, Gauduin MC, Paiardini M, McClure HM, Johnson RP, Silvestri G, Sodora DL
Blood, 2003-10-02;103(3):973-9.
Species: Primate - Cebus capucinus (White-headed Capuchin Monkey), Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Plasma
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Interleukin-7 improves CD4 T-cell reconstitution after autologous CD34 cell transplantation in monkeys.
Authors: Storek J, Gillespy T, Lu H, Joseph A, Dawson MA, Gough M, Morris J, Hackman RC, Horn PA, Sale GE, Andrews RG, Maloney DG, Kiem HP
Blood, 2003-01-23;101(10):4209-18.
Species: Primate - Papio cynocephalus (Yellow Baboon)
Sample Types: Serum
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