Human Importin alpha 3/KPNA4 Antibody

Catalog #: MAB8204 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8204
MAB8204-SP

Detection of Human Importin alpha 3/KPNA4 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Importin alpha 3/KPNA4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Importin alpha 3/KPNA4 in ELISA and Western blot. In direct ELISA, no cross-reactivity to human KPNA3 was detected.

Source

Monoclonal Mouse IgG_2B Clone # 843124

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Importin alpha 3/KPNA4
Met1-Asn104
Accession # O00629

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Importin alpha 3/KPNA4 by Western Blot. Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Importin a3/KPNA4 Monoclonal Antibody (Catalog # MAB8204) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Importin a3/KPNA4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human Importin alpha 3/KPNA4 by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Importin alpha 3/KPNA4 at approximately 56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Importin alpha 3/KPNA4 Monoclonal Antibody (Catalog # MAB8204). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Importin alpha 3/KPNA4

KPNA4 (Karyopherin subunit alpha 4), also called Importin subunit a4 or Importin aQ1 (previously called Qip1 or Importin a3) is an approximately 58-60 kDa member of the Importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. KPNA4 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. Human KPNA4 is a 521 amino acid (aa) protein that contains an N-terminal IBB/Importin b domain (aa 2-58), ten Armadillo repeats that bind “cargo” (aa 66-485) and two intervening NLS binding sites. Human KPNA4 aa 1-104 share 99% aa identity with mouse and rat KPNA4, which in turn share 100% identity with each other.

Long Name

Karyopherin alpha 4

Entrez Gene IDs

3840 (Human); 16649 (Mouse); 361959 (Rat)

Alternate Names

Importin alpha 3; Importin alpha Q1; importin subunit alpha-4; importin-alpha-Q1; IPOA3; karyopherin alpha 4 (importin alpha 3); KPNA4; MGC12217; MGC26703; Qip1; QIP12; QIP1Karyopherin subunit alpha-4; SRP3