Human Importin alpha 5/KPNA1 Antibody

Catalog #: AF6206 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6206
AF6206-SP

Detection of Human Importin alpha 5/KPNA1 by Western Blot.

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Human Importin alpha 5/KPNA1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Importin alpha 5/KPNA1 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Importin alpha 5/KPNA1
Met1-Val132
Accession # P52294

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Importin alpha 5/KPNA1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Importin a5/KPNA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6206) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Importin a5/KPNA1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Importin alpha 5/KPNA1

Importin subunit alpha-5 (KPNA1, Karyopherin subunit alpha 1; also SRP1 beta, NPI-1 and RAG2) is a 65 kDa member of the importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. KPNA1 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. Two scenarios are possible. First, NLS‑containing cargo molecules bind to KPNA1, which then binds to importin beta ; alternatively, KPNA1 and importin beta initially form a complex that primes KPNA1 for subsequent cargo binding. In either case, importin beta binds to the nuclear pore, facilitating transport into the nucleus. Human KPNA1 is 538 amino acids (aa) in length. It contains an N-terminal IBB/importin beta domain (aa 1‑57), ten Armadillo repeats that bind "cargo" (aa 77‑504) and two intervening NLS binding sites. Over aa 1-132, human KPNA1 shares 97% aa identity with mouse KPNA1.

Entrez Gene IDs

3836 (Human); 16646 (Mouse); 288064 (Rat)

Alternate Names

Importin alpha 5; importin subunit alpha-1; importin-alpha-S1; IPOA5; karyopherin alpha 1 (importin alpha 5); Karyopherin subunit alpha-1; KPNA1; NPI-1; NPI-1SRP1importin alpha 5; Nucleoprotein interactor 1; RCH2; RCH2RAG cohort protein 2; recombination activating gene cohort 2; SRP1; SRP1-beta