Human iNOS Quantikine ELISA Kit

Discontinued Product

DNS00 has been discontinued.
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R&D Systems Quantikine ELISAs
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Citations (4)
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Human iNOS Quantikine ELISA Kit Summary

Sensitivity
0.46 pg/mL
Assay Range
1.3 - 80 pg/mL
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: iNOS

Nitric oxide (NO) is a pleiotropic signaling molecule implicated in diverse biological processes including inhibition of platelet aggregation, regulation of neurotransmission, vasodilation, immune responses, and inflammation. NO is synthesized from L-arginine and O2 by three nitric oxide synthase (NOS) enzymes termed endothelial NOS (eNOS), neuronal NOS (nNOS), and inducible NOS (iNOS). Each enzyme isoform is expressed in a variety of tissues and cell types. While eNOS and nNOS generally exhibit constitutive expression and are involved in physiological signaling and maintenance functions, iNOS expression is induced by inflammatory stimuli and is associated with both normal and pathological immune responses.

Long Name:
Inducible Nitic Oxide Synthase
Entrez Gene IDs:
4843 (Human); 18126 (Mouse)
Alternate Names:
EC 1.14.13.39; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; nitric oxide synthase 2, inducible; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase, inducible; nitric oxide synthase, macrophage; NOS type II; NOS; NOS2; NOS2A; Peptidyl-cysteine S-nitrosylase NOS2

Citations for Human iNOS Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Nitric oxide attenuates vascular endothelial cadherin-mediated vascular integrity in human chronic inflammation.
    Authors: Hama S, Takeichi O, Fujisaki K, Tanabe N, Maeno M, Ochiai K
    Clin. Exp. Immunol., 2008-12-01;154(3):384-90.
    Species: Human
    Sample Types: Cell Lysates
  2. Time-dependent expression of renal vaso-regulatory molecules in LPS-induced endotoxemia in rat.
    Authors: Yamaguchi N, Jesmin S, Zaedi S, Shimojo N, Maeda S, Gando S, Koyama A, Miyauchi T
    Peptides, 2006-05-24;27(9):2258-70.
    Species: Rat
    Sample Types: Tissue Homogenates
  3. Neuropeptide (calcitonin gene-related peptide) induction of nitric oxide in human keratinocytes in vitro.
    Authors: E Y, Golden SC, Shalita AR, Lee WL, Maes DH, Matsui MS
    J. Invest. Dermatol., 2006-05-18;126(9):1994-2001.
    Species: Human
    Sample Types: Cell Lysates
  4. Combined effect of testosterone and apocynin on nitric oxide and superoxide production in PMA-differentiated THP-1 cells.
    Authors: Juliet PA, Hayashi T, Daigo S, Matsui-Hirai H, Miyazaki A, Fukatsu A, Funami J, Iguchi A, Ignarro LJ
    Biochim. Biophys. Acta, 2004-09-17;1693(3):185-91.
    Species: Human
    Sample Types: Cell Lysates

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