Human Integrin alpha 10 Antibody

Name: Human Integrin alpha 10 Antibody
Brand: R&D Systems
SKU: AF4579

Catalog #: AF4579 Datasheet / COA / SDS

Discontinued Product

AF4579 has been discontinued.

View all Integrin alpha 10 products.

Detection of Integrin alpha 10 in SW1353 Human Cell Line by Flow Cytometry.

1 Image

All Integrin alpha 10 Antibodies

Product Details

FAQs

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin alpha 10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin alpha 10 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Integrin alpha 1, rhIntegrin alpha 2, and rhIntegrin alpha 11 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Integrin alpha 10
Thr685-Glu831
Accession # O75578

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of Integrin alpha 10 in SW1353 Human Cell Line by Flow Cytometry. SW1353 human chondrosarcoma cell line was stained with Sheep Anti-Human Integrin a10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4579, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 10

Integrin alpha 10 (also ITGA10) is a 160 kDa member of the integrin alpha chain family of molecules. Mature human integrin alpha 10 is a 1145 amino acid (aa) type I transmembrane glycoprotein that contains a 1100 aa extracellular domain (ECD) (aa 23-1122) plus a 22 aa cytoplasmic tail. The ECD contains two FG-GAP regions (aa 24-95), followed by one vWFA domain (aa167-350) and five consecutive FG-GAP regions (aa 361-657). The FG-GAP repeats are suggested to participate in the formation of a beta -propeller. Integrin alpha 10 is expressed by chondrocytes in hyaline cartilage, and mesenchymal stem cells with chondrogenic potential. It is also synthesized by fibroblasts in epimysium and tendon. Integrin alpha 10 forms a nondisulfide-linked heterodimer with integrin beta 1, and this complex serves as a receptor for collagen type II. There are multiple potential isoform variants. One reportedly contains a Val-Ser substitution for aa 123-1167, a second shows a deletion of aa 19-161, and a third possesses a 30 aa substitution for aa 1-161. Over aa 685-831, human Integrin alpha 10 shares 86% aa sequence identity with mouse integrin alpha 10.

Entrez Gene IDs

8515 (Human); 213119 (Mouse); 310683 (Rat)

Alternate Names

Integrin alpha 10; integrin alpha-10; integrin, alpha 10; ITGA10; PRO827