Human Integrin alpha 2/CD49b PE-conjugated Antibody
Human Integrin alpha 2/CD49b PE-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Integrin alpha 2/CD49b in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin a2/CD49b PE-conjugated Monoclonal Antibody (Catalog # FAB1233P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
Background: Integrin alpha 2/CD49b
Integrin alpha 2 is one of twelve integrin family alpha subunits that share the beta 1 subunit (1‑3). Integrin alpha 2 beta 1 is the non-covalent heterodimer of 160 kDa alpha 2 (CD49b) and 130 kDa beta 1 (CD29) type I transmembrane glycoprotein subunits and is one of six very late antigens on activated T cells, designated VLA2 (3). The alpha 2 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1, 2). The 1102 amino acid (aa) human alpha 2 extracellular domain (ECD) shares 83‑89% aa sequence identity with mouse, rat, canine, bovine and equine alpha 2. The I domain-containing beta 1 integrins ( alpha 1 beta 1, alpha 2 beta 1, alpha 10 beta 1 and alpha 11 beta 1) all bind collagens, with alpha 2 beta 1 preferring collagens I‑III (4, 5). Platelet alpha 2 beta 1, also called GPIa, cooperates with another adhesion protein, GPVI, to coordinate platelet collagen binding and activation (3, 6, 7). Other alpha 2 beta 1 ligands include laminin, decorin, E-cadherin, and collagen-like regions of collectin molecules such as C1q (4). Adhesion is synergized by crosstalk with syndecan-1 or HGF R/c-Met, and antagonized by crosstalk with Integrin alpha 1 beta 1 (8‑10). In addition to expression on selected hematopoietic cells, alpha 2 beta 1 is present on a wide variety of non-hematopoietic cells (4). Mice deficient in the alpha 2 subunit have defects in innate immune responses, wound mast cell infiltration and angiogenesis, and platelet responses to collagen (6, 11, 12). In innate immunity, alpha 2 beta 1 binding to C1q initiates the complement cascade and costimulates mast cell activation, triggering neutrophil influx (4, 12).
- Takada, Y. et al. (2007) Genome Biol. 8:215.
- Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
- Takada, Y. and M.E. Hemler (1989) J. Cell Biol. 109:397.
- Zutter, M.M. and B.T Edelson (2007) Immunobiology 212:343.
- McCall-Culbreath, K.D. and M.M. Zutter (2008) Curr. Drug Targets 9:139.
- Sarratt, K.L. et al. (2005) Blood 106:1268.
- Lecut, C. et al. (2005) Thromb. Haemost. 94:107.
- Vuoriluoto, K. et al. (2008) Exp. Cell Res. 314:3369.
- McCall-Culbreath, K.D. et al. (2008) Blood 111:3562.
- Abair, T.D. et al. (2008) Exp. Cell Res. 314:3593.
- Zweers, M. et al. (2006) J. Invest. Dermatol. 127:467.
- Edelson, B.T. et al. 2006) Blood 107:143.
Product Datasheets
Citations for Human Integrin alpha 2/CD49b PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
3
Citations: Showing 1 - 3
Filter your results:
Filter by:
-
Single-Cell RNA Sequencing Reveals Distinct Cardiac-Derived Stromal Cell Subpopulations
Authors: JR Hoffman, AR Jayaraman, S Bheri, ME Davis
Journal of cardiovascular development and disease, 2022-11-01;9(11):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Capturing human trophoblast development with naive pluripotent stem cells in�vitro
Authors: S Io, M Kabata, Y Iemura, K Semi, N Morone, A Minagawa, B Wang, I Okamoto, T Nakamura, Y Kojima, C Iwatani, H Tsuchiya, B Kaswandy, E Kondoh, S Kaneko, K Woltjen, M Saitou, T Yamamoto, M Mandai, Y Takashima
Cell Stem Cell, 2021-04-07;28(6):1023-1039.e13.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium.
Authors: Albert R, Vereb Z, Csomos K, Moe M, Johnsen E, Olstad O, Nicolaissen B, Rajnavolgyi E, Fesus L, Berta A, Petrovski G
PLoS ONE, 2012-10-09;7(10):e47187.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human Integrin alpha 2/CD49b PE-conjugated Antibody
There are currently no reviews for this product. Be the first to review Human Integrin alpha 2/CD49b PE-conjugated Antibody and earn rewards!
Have you used Human Integrin alpha 2/CD49b PE-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
