Human Integrin alpha 3/CD49c Biotinylated Antibody

Catalog #: BAM1345
1 Citation Datasheet / COA / SDS

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BAM1345

Integrin alpha 3/CD49c in HT1080 Human Cell Line.

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Human Integrin alpha 3/CD49c Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin alpha 3/CD49c.

Source

Monoclonal Mouse IgG₁ Clone # IA3

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human milk epithelial cell line

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

HT1080 human fibrosarcoma cell line

Immunocytochemistry

8-25 µg/mL

Immersion fixed HT1080 human fibrosarcoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Integrin alpha 3/CD49c in HT1080 Human Cell Line. Integrin a3/CD49c was detected in immersion fixed HT1080 human fibrosarcoma cell line using Mouse Anti-Human Integrin a3/CD49c Biotinylated Monoclonal Antibody (Catalog # BAM1345) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 3/CD49c

The alpha 3 subunit, also known as CD49c and VLA-3 alpha subunit, forms a non-covalent heterodimer with the Integrin beta 1 subunit (CD29). Integrin alpha 3 beta 1 is a receptor for laminin, fibronectin, collagen, epiligrin, and thrombospondin.

Entrez Gene IDs

3675 (Human); 16400 (Mouse)

Alternate Names

antigen identified by monoclonal J143; CD49 antigen-like family member C; CD49c antigen; CD49c; FLJ34631; FLJ34704; FRP-2; Galactoprotein B3; GAP-B3; GAPB3CD49C; Integrin alpha 3; integrin alpha-3; integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor); ITGA3; MSK18; VCA-2; very late activation protein 3 receptor, alpha-3 subunit; VL3A; VLA-3 subunit alpha; VLA3a

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human Integrin alpha 3/CD49c Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Glycosylation modulates melanoma cell alpha2beta1 and alpha3beta1 integrin interactions with type IV collagen.
Authors: Stawikowski M, Aukszi B, Stawikowska R, Cudic M, Fields G
J Biol Chem, 2014-06-23;289(31):21591-604.
Species: Human
Sample Types: Whole Cells
Applications: ICC