Human Integrin alpha 4/CD49d APC-conjugated Antibody

Name: Human Integrin alpha 4/CD49d APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB1354A

Catalog #: FAB1354A
1 Citation Datasheet / COA / SDS

Discontinued Product

FAB1354A has been discontinued.

View all Integrin alpha 4/CD49d products.

Detection of Integrin alpha 4/CD49d in Human Blood Lymphocytes by Flow Cytometry.

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All Integrin alpha 4/CD49d Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin alpha 4/CD49d APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects the human Integrin alpha 4/CD49d subunit in flow cytometry.

Source

Monoclonal Mouse IgG₁ Clone # 7.2R

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

DX-3 human melanoma cell line

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Integrin alpha 4/CD49d in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human Integrin a4/CD49d APC-conjugated Monoclonal Antibody (Catalog # FAB1354A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Integrin alpha 4/CD49d

The Integrin alpha 4 subunit, also known as CD49d and VLA-4 alpha subunit, forms heterodimers with Integrin beta 1 (CD29) or beta 7. Integrins alpha 4 beta 1 (VLA4) and alpha 4 beta 7 are both receptors for fibronectin and VCAM. Integrin alpha 4 beta 7 also binds the mucosal addressin cell adhesion molecule (MAdCAM-1). On the cell surface, VLA4 will participate in homotypic binding between endothelial cells and leukocytes. The extracellular domains of human and mouse Integrin alpha 4 share 85% aa sequence identity.

Entrez Gene IDs

3676 (Human); 16401 (Mouse); 102128433 (Cynomolgus Monkey)

Alternate Names

269C wild type; CD49 antigen-like family member D; CD49d antigen; CD49d; CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor; IA4; Integrin alpha 4; integrin alpha-4 subunit; integrin alpha-4; Integrin alpha-IV; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); ITGA4; MGC90518; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha; VLA-4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Integrin alpha 4/CD49d APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.
Authors: Shantsila E, Wrigley B, Tapp L, Apostolakis S, Montoro-Garcia S, Drayson MT, Lip GY
J. Thromb. Haemost., 2011-05-01;9(5):1056-66.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry