Home / Integrin alpha 8 / Human Integrin  alpha 8 Antibody

Human Integrin  alpha 8 Antibody

Catalog #: MAB6194
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6194
MAB6194-SP
Detection of Integrin alpha 8 in H4 Human Cell Line by Flow Cytometry.
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Product Details
Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin  alpha 8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha 8 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 481709
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Integrin alpha 8
Glu610-Leu800
Accession # P53708
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin a8 antibody in H4 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Integrin alpha 8 in H4 Human Cell Line by Flow Cytometry. H4 human neuroglioma cell line was stained with Mouse Anti-Human Integrin a8 Monoclonal Antibody (Catalog # MAB6194, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Immunocytochemistry Integrin a8 antibody in H4 Human Cell Line by Immunocytochemistry (ICC). View Larger

Integrin alpha 8 in H4 Human Cell Line. Integrin a8 was detected in immersion fixed H4 human neuroglioma cell line using Mouse Anti-Human Integrin a8 Monoclonal Antibody (Catalog # MAB6194) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 8

Integrin alpha 8 is a 170-200 kDa member of the Integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with Integrin beta 1. Integrin alpha 8/ beta 1 promotes both cell adhesion and survival, and is known to bind to fibronectin, LAP-TGF beta 1 and Nephronectin. It is principally expressed in smooth muscle cells.  Human alpha 8 is a 1025 amino acid (aa) type I transmembrane glycoprotein. It contains a 974 aa extracellular domain (ECD) (aa 39-1012) and a 30 aa cytoplasmic domain. In the ECD, human alpha 8 shares 90% aa sequence identity with mouse alpha 8 protein.

Entrez Gene IDs
8516 (Human); 241226 (Mouse); 84381 (Rat)
Alternate Names
Integrin alpha 8; integrin alpha-8; integrin, alpha 8; ITGA8

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Citations for Human Integrin  alpha 8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Single-molecule characterization of subtype-specific beta1 integrin mechanics
    Authors: MH Jo, J Li, V Jaumouillé, Y Hao, J Coppola, J Yan, CM Waterman, TA Springer, T Ha
    Nature Communications, 2022-12-03;13(1):7471.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Enteric Species F Human Adenoviruses use Laminin-Binding Integrins as Co-Receptors for Infection of Ht-29 Cells
    Authors: A Rajan, BD Persson, L Frängsmyr, A Olofsson, L Sandblad, J Heino, Y Takada, AP Mould, LM Schnapp, J Gall, N Arnberg
    Sci Rep, 2018-07-03;8(1):10019.
    Applications: Western Blot
  3. 9-cis-retinoic Acid and troglitazone impacts cellular adhesion, proliferation, and integrin expression in K562 cells.
    Authors: Hanson A, Gambill J, Phomakay V, Staten C, Kelley M
    PLoS ONE, 2014-03-26;9(3):e93005.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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