Human Integrin  alpha L/CD11a Antibody

Detection of Integrin  alpha L/CD11a in Human Spleen. Integrin  alpha L/CD11a was detected in immersion fixed paraffin-embedded sections of Human Spleen using Mouse Anti-Human Integrin  alpha L/CD11a Monoclonal Antibody (Catalog # MAB35952) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human Integrin  alpha L/CD11a by Western Blot. Western blot shows lysates of Jurkat Human Acute T Cell Leukemia Cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Integrin  alpha L/CD11a Monoclonal Antibody (Catalog # MAB35952) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Integrin  alpha L/CD11a at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human Integrin  alpha L/CD11a by Simple Western^TM. Simple Western lane view shows lysates of Human PBMCs, loaded at 0.2 mg/mL. A specific band was detected for Integrin  alpha L/CD11a at approximately 190 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human Integrin  alpha L/CD11a Monoclonal Antibody (Catalog # MAB35952). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.