Human Integrin alpha L/CD11a Fluorescein‑conjugated Antibody

Name: Human Integrin alpha L/CD11a Fluorescein‑conjugated Antibody
Brand: R&D Systems
SKU: FAB3595F
Price: 339 USD
Availability: InStock

R&D Systems | Catalog # FAB3595F

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Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG₁ Clone # 345913

View all Integrin alpha L/CD11a Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha L/CD11a
Tyr26-Met1089 (Tyr660Ile)
Accession # CAA68747

Specificity

Detects human Integrin alpha L/CD11a in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Integrin alpha L/CD11a Fluorescein‑conjugated Antibody

Detection of Integrin alpha L/CD11a in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human Integrin aL/CD11a Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3595F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin alpha L/CD11a Fluorescein‑conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood lymphocytes

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha L/CD11a

Integrin subunit alpha L/CD11a is a 180 kDa type I TM glycoprotein that interacts only with Integrin beta 2/CD18 to form LFA-1, a leukocyte adhesion protein which binds endothelial cell ICAM-1 through -4. Human Integrin alpha L contains a 1064 aa extracellular domain (ECD), a 20 aa TM sequence and a 58 aa cytoplasmic domain. The ECD contains seven repeats that form a beta-propeller structure and one inserted vWA domain (I domain) containing a metal ion-dependent adhesion site (MIDAS). Human and mouse Integrin alpha L ECD share 74% aa identity. CD11a has two splice variants, one which contains a 53 aa insert after Gln954, and a second which shows a deletion of aa 110-192.