Human Integrin alpha L/CD11a Fluorescein-conjugated Antibody

Catalog #: FAB3595F Datasheet / COA / SDS

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FAB3595F

Detection of Integrin alpha L/CD11a in Human Blood Lymphocytes by Flow Cytometry.

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Human Integrin alpha L/CD11a Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin alpha L/CD11a in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 345913

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha L/CD11a
Tyr26-Met1089 (Tyr660Ile)
Accession # CAA68747

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Integrin alpha L/CD11a in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human Integrin aL/CD11a Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3595F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha L/CD11a

Integrin subunit alpha L/CD11a is a 180 kDa type I TM glycoprotein that interacts only with Integrin beta 2/CD18 to form LFA-1, a leukocyte adhesion protein which binds endothelial cell ICAM-1 through -4. Human Integrin alpha L contains a 1064 aa extracellular domain (ECD), a 20 aa TM sequence and a 58 aa cytoplasmic domain. The ECD contains seven repeats that form a beta-propeller structure and one inserted vWA domain (I domain) containing a metal ion-dependent adhesion site (MIDAS). Human and mouse Integrin alpha L ECD share 74% aa identity. CD11a has two splice variants, one which contains a 53 aa insert after Gln954, and a second which shows a deletion of aa 110-192.

Entrez Gene IDs

3683 (Human); 16408 (Mouse); 308995 (Rat)

Alternate Names

CD11 antigen-like family member A; CD11a antigen; CD11a; CD11Aantigen CD11A (p180), lymphocyte function-associated antigen 1, alphapolypeptide; Integrin alpha L; integrin alpha-L; integrin gene promoter; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen1; alpha polypeptide); ITGAL; Leukocyte adhesion glycoprotein LFA-1 alpha chain; Leukocyte function-associated molecule 1 alpha chain; LFA-1 alpha; LFA-1; LFA1A; LFA-1A; Ly-15; Ly-21; lymphocyte function-associated antigen 1