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Human Integrin  beta 2/CD18 Antibody

Catalog #: AF1730
16 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1730
AF1730-SP
Integrin beta 2/CD18 in Human PBMCs.
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Citations (16)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin  beta 2/CD18 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin beta 2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Integrin  beta 2
Gln23-Asn700
Accession # AAA59490
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood mononuclear cells
Adhesion Blockade
The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized Recombinant Human ICAM-1/CD54 Fc Chimera (Catalog # 720-IC, 12.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 20 µg/mL of the antibody.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
Immersion fixed human peripheral blood mononuclear cells (PBMCs), THP‑1 human acute monocytic leukemia cells (Positive) & absent in RT‑4 human urinary bladder transitional cell papilloma cells (Negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Integrin beta 2/CD18 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

Integrin beta 2/CD18 in Human PBMCs. Integrin beta 2/CD18 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human Integrin beta 2/CD18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1730) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry View Larger

Detection of Integrin  beta 2/CD18 in THP‑1 cells (Positive) & absent in RT‑4 cells (Negative). Integrin  beta 2/CD18 was detected in immersion fixed THP‑1 human acute monocytic leukemia cells (Positive) & absent in RT‑4 human urinary bladder transitional cell papilloma (Negative) using Goat Anti-Human Integrin  beta 2/CD18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1730) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to Cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 2/CD18

Integrin beta 2 is a transmembrane protein expressed by all leukocytes. It occurs in heterodimeric complexes with Integrin subunits alpha D, alpha L (complex also known as LFA‑1), alpha M (Mac-1), or alpha X (CR-4). These complexes are important in adhesion and leukocyte trafficking.

Entrez Gene IDs
3689 (Human); 16414 (Mouse); 309684 (Rat); 101925124 (Cynomolgus Monkey)
Alternate Names
959 beta subunit precursor)10; CD18 antigen; CD18; CD18beta 2; cell surface adhesion glycoprotein (LFA-1; CR3; Integrin beta 2; integrin beta-2; integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); ITGB2; LAD; LCAMB; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; LFA-1 beta; LFA-1; MAC-1 beta; MF17; MFI7; P150; p150,95 beta

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Citations for Human Integrin  beta 2/CD18 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Identification of the Tetraspanin CD82 as a New Barrier to Xenotransplantation
    Authors: Soad M. Saleh, Ranjit S. Parhar, Reem S. Al-Hejailan, Razan H. Bakheet, Hala S. Khaleel, Hanif G. Khalak et al.
    The Journal of Immunology
  2. Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters
    Authors: Stempels FC, Jiang M, Warner HM et al.
    The Journal of cell biology
  3. PAI-1 uncouples integrin-?1 from restrain by membrane-bound ?-catenin to promote collagen fibril remodeling in obesity-related neoplasms
    Authors: Lin, LL;Nayak, B;Osmulski, PA;Wang, E;Wang, CP;Valente, PT;Wang, CM;Tan, X;Santanam, N;Wang, TL;Gaczynska, ME;Kost, ER;Huang, TH;Kirma, NB;
    Cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  4. Leishmania braziliensis enhances monocyte responses to promote anti-tumor activity
    Authors: Dos Santos, JC;Moreno, M;Teufel, LU;Chilibroste, S;Keating, ST;Groh, L;Domínguez-Andrés, J;Williams, DL;Ma, Z;Lowman, DW;Ensley, HE;Novakovic, B;Ribeiro-Dias, F;Netea, MG;Chabalgoity, JA;Joosten, LAB;
    Cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  5. The metabolism of cells regulates their sensitivity to NK cells depending on p53 status
    Authors: S Belkahla, JM Brualla, A Fayd'herbe, P Falvo, N Allende-Ve, M Constantin, AUH Khan, L Coenon, C Alexia, G Mitola, P Massa, S Orecchioni, F Bertolini, W Mnif, J Hernandez, A Anel, M Villalba
    Scientific Reports, 2022-02-25;12(1):3234.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Metformin sensitizes leukemic cells to cytotoxic lymphocytes by increasing expression of intercellular adhesion molecule-1 (ICAM-1)
    Authors: N Allende-Ve, J Marco Brua, P Falvo, C Alexia, M Constantin, AF de Maudave, L Coenon, D Gitenay, G Mitola, P Massa, S Orecchioni, F Bertolini, I Marzo, A Anel, M Villalba
    Scientific Reports, 2022-01-25;12(1):1341.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Hepatocytes Delete Regulatory T Cells by Enclysis, a CD4+ T Cell Engulfment Process
    Authors: SP Davies, GM Reynolds, AL Wilkinson, X Li, R Rose, M Leekha, YS Liu, R Gandhi, E Buckroyd, J Grove, NM Barnes, RC May, SG Hubscher, DH Adams, Y Huang, O Qureshi, Z Stamataki
    Cell Rep, 2019-11-05;29(6):1610-1620.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: IF
  8. The Extracellular Domain of the β2 Integrin β Subunit (CD18) Is Sufficient for Escherichia coli Hemolysin and Aggregatibacter actinomycetemcomitans Leukotoxin Cytotoxic Activity
    Authors: LC Ristow, V Tran, KJ Schwartz, L Pankratz, A Mehle, JD Sauer, RA Welch
    MBio, 2019-07-09;10(4):.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  9. Hypoxia and matrix viscoelasticity sequentially regulate endothelial progenitor cluster-based vasculogenesis
    Authors: MR Blatchley, F Hall, S Wang, HC Pruitt, S Gerecht
    Sci Adv, 2019;5(3):eaau7518.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  10. Biomimetic post-capillary venule expansions for leukocyte adhesion studies
    Authors: BL Benson, L Li, JT Myers, RD Dorand, UA Gurkan, AY Huang, RM Ransohoff
    Sci Rep, 2018-06-19;8(1):9328.
  11. Synthesis of Human Neutrophil Extracellular Traps Contributes to Angiopoietin-Mediated In Vitro Proinflammatory and Proangiogenic Activities
    Authors: SS Lavoie, E Dumas, B Vulesevic, PE Neagoe, M White, MG Sirois
    J. Immunol., 2018-04-23;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Bystander cells enhance NK cytotoxic efficiency by reducing search time
    Authors: X Zhou, R Zhao, K Schwarz, M Mangeat, EC Schwarz, M Hamed, I Bogeski, V Helms, H Rieger, B Qu
    Sci Rep, 2017-03-13;7(0):44357.
    Species: Human
    Sample Types: Whole Cells
    Applications: Migration Assay
  13. An anti-inflammatory property of Candida albicans beta-glucan: Induction of high levels of interleukin-1 receptor antagonist via a Dectin-1/CR3 independent mechanism.
    Authors: Smeekens S, Gresnigt M, Becker K, Cheng S, Netea S, Jacobs L, Jansen T, van de Veerdonk F, Williams D, Joosten L, Dinarello C, Netea M
    Cytokine, 2014-11-20;71(2):215-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Mesenchymal stem cell contact promotes CCN1 splicing and transcription in myeloma cells.
    Authors: Dotterweich J, Ebert R, Kraus S, Tower R, Jakob F, Schutze N
    Cell Commun Signal, 2014-06-25;12(0):36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. Complement Receptor Mac-1 Is an Adaptor for NB1 (CD177)-mediated PR3-ANCA Neutrophil Activation.
    Authors: Jerke U, Rolle S, Dittmar G, Bayat B, Santoso S, Sporbert A, Luft F, Kettritz R
    J. Biol. Chem., 2010-12-30;286(9):7070-81.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
    Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
    Dig. Dis. Sci., 2007-03-30;52(10):2775-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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