Human IRAK4 Antibody

Detection of Human IRAK4 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human IRAK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3919) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). For additional reference, recombinant human IRAK1, IRAK2, and IRAK4 (2 ng/lane) were included. A specific band for IRAK4 was detected at approxi­mately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IRAK4 in THP‑1 Human Cell Line. IRAK4 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Goat Anti-Human IRAK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3919) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Human IRAK4 by Simple Western^TM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and THP‑1 human acute monocytic leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for IRAK4 at approximately 55 kDa (as indicated) using 20 µg/mL of Goat Anti-Human IRAK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3919). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.