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Human IRF5 PerCP-conjugated Antibody

Catalog #: IC4508C Datasheet / COA / SDS

Discontinued Product

IC4508C has been discontinued.
View all IRF5 products.
Detection of IRF5 in Human PBMCs by Flow Cytometry.
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Human IRF5 PerCP-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IRF5 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IRF5
Ala214-Thr395
Accession # NP_002191
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IRF5 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of IRF5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Sheep Anti-Human IRF5 PerCP-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC4508C, filled histogram) or isotype control antibody (Catalog # IC016C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IRF5

IRF5 is a member of the IRF family of transcription factors, a family characterized by a helix-turn-helix DNA binding domain enriched in tryptophan repeats. IRF family members show diverse cellular regulation of interferon-stimulated gene transcription, viral-mediated gene activation, apoptosis, differentiation, and cellular growth. IRF5, along with IRF7, are the key mediators of TLR signaling. IRF5 forms heterodimers with IRF3 both of which are necessary for interferon gene transcription. IRF5 knock out mice indicate that IRF5 is critical for induction of apoptosis.

Long Name
Interferon Regulatory Factor 5
Entrez Gene IDs
3663 (Human); 27056 (Mouse); 296953 (Rat)
Alternate Names
IBD14; interferon regulatory factor 5; IRF5; IRF-5; SLEB10

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