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Human iRHOM2/RHBDF2 Antibody

Catalog #: MAB10048
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB10048-100
MAB10048-SP
Detection of Human iRHOM2/RHBDF2 by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human iRHOM2/RHBDF2 Antibody Summary

Species Reactivity
Human
Specificity
Detects huma iRHOM2/RHBDF2 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2A Clone # 996308
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Synthetic peptide containing human iRHOM2/RHBDF2
Accession # Q6PJF2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human iRHOM2/RHBDF2 antibody by Western Blot. View Larger

Detection of Human iRHOM2/RHBDF2 by Western Blot. Western blot shows lysates of LoVo human colorectal adenocarcinoma cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human iRHOM2/RHBDF2 Monoclonal Antibody (Catalog # MAB10048) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for iRHOM2/RHBDF2 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: iRHOM2/RHBDF2

Human Inactive rhomboid protein 2 (RHBDF2 or IRHOM2) is a highly conserved seven-transmembrane Rhomboid protease-like protein which is inactive, it has no protease activity, but has been implicated in the secretion of several ligands of the epidermal growth factor receptor (EGFR), probably by regulating trafficking and stability of key protein membrane like ADAM17 and STING. The RHBDF2 protein plays an important role in the secretion of tumor necrosis factor alpha and has been implicated in familial esophageal cancer. Gain-of-function (GOF) mutations in the RHBDF2 gene constitutively activate EGFR signaling to cause tylosis with esophageal cancer syndrome.

Long Name
Rhomboid 5 Homolog 2
Entrez Gene IDs
79651 (Human); 217344 (Mouse); 303690 (Rat)
Alternate Names
FLJ22341; iRHOM2; RHBDF2; RHBDL5; RHBDL5rhomboid family member 2; RHBDL6; rhomboid 5 homolog 2 (Drosophila); Rhomboid 5 homolog 2; rhomboid veinlet-like 5; Rhomboid veinlet-like protein 5; Rhomboid veinlet-like protein 6; rhomboid, veinlet-like 6 (Drosophila); rhomboid, veinlet-like 6; rhomboid-like protein 6; TOCG

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Citations for Human iRHOM2/RHBDF2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Ectodomain Shedding by ADAM17 Increases the Release of Soluble CD40 from Human Endothelial Cells under Pro-Inflammatory Conditions
    Authors: Klersy, A;Meyer, S;Leuschner, F;Kessler, T;Hecker, M;Wagner, AH;
    Cells
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  2. Inflammatory activation of surface molecule shedding by upregulation of the pseudoprotease iRhom2 in colon epithelial cells
    Authors: AA Giese, A Babendreye, P Krappen, A Gross, P Strnad, S Düsterhöft, A Ludwig
    Scientific Reports, 2021-12-20;11(1):24230.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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