Human Irisin/FNDC5 Antibody

Catalog #: MAB94202 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB94202-100
MAB94202-SP

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Detection of Irisin/FNDC5 in Human Heart.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Irisin/FNDC5 Antibody Summary

Specificity

Detects Irisin peptide in direct ELISA.

Source

Monoclonal Mouse IgG_2A Clone # 1056126

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide covering the C-term of the Irisin peptide sequences of the FNDC5 gene.
Glu97-Glu143
Accession # Q8NAU1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of Human Heart.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of Irisin/FNDC5 in Human Heart. Irisin/FNDC5 was detected in immersion fixed paraffin-embedded sections of Human Heart using Mouse Anti-Human Irisin/FNDC5 Monoclonal Antibody (Catalog # MAB94202) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intercalated disks. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Irisin/FNDC5

Irisin (also known as FNDC5) is a 12 kDa glycosylated polypeptide hormone that regulates energy metabolism, stem cell differentiation, and neuronal development (1, 2). Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation (2-5). Mature human, mouse, and rat Irisin share 100% sequence identity. Expression of Irisin is induced in skeletal muscle and subcutaneous adipose tissue during and shortly after exercise (2, 6). Irisin induces expression of peroxisome proliferator-activated receptor gamma co-activator 1 alpha (PGC1 alpha ) and uncoupling protein-1 (UCP1), mitochondrial-associated metabolic proteins (7, 8). Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue. In mice, expression of Irisin has been shown to regulate obesity and diabetes (1, 2). A similar function in humans is suggested (9). Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts (10-14).

References

Bostrom, P. et al. (2012) Nature 481:463.
Novelle, M.G. et al. (2013) Int. J. Endocrinol. 2013:746281.
Ferrer-Martinez, A. et al. (2002) Dev. Dyn. 224:154.
Teufel, A. et al. (2002) Gene 297:79.
Zhang, W. et al. (2015) Neurosci. Lett. 595:7.
Roca-Rivada, A. et al. (2013) PLoS One 8:e60563.
Vaughan, R.A. et al. (2014) Diabetes Obes. Metab. 16:711.
Jeong Lee, H. et al. (2015) Mol. Endocrinol. 6:873.
Elsen, M. et al. (2014) J. Endocrinol. 222:R25.
Dun, S.L. et al. (2013) Neuroscience 240:155.
Forouzanfar, M. et al. (2015) Cell. Biol. Int. 39:629.
Hashemi, M.S. et al. (2013) Neuroscience 231:296.
Moon, H.S. et al. (2013) Metabolism 62:1131.
Colaianni, G. et al. (2014) Int. J. Endocrinol. 2014:902186.

Long Name

Fibronectin Type III Domain Containing 5

Entrez Gene IDs

252995 (Human); 384061 (Mouse); 260327 (Rat)

Alternate Names

fibronectin type III domain containing 5; fibronectin type III domain-containing protein 5; FNDC5; FRCP2; FRCP2Fibronectin type III repeat-containing protein 2; Irisin