Human Irisin/FNDC5 Antibody Summary
Asp32-Glu143
Accession # Q8NAU1
Applications
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Irisin/FNDC5 Monoclonal Antibody (Catalog # MAB8880).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Irisin/FNDC5 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocarcinoma cell line, cultured in media containing low glucose and 100 nM insulin, was stained with Mouse Anti-Human Irisin/FNDC5 Monoclonal Antibody (Catalog # MAB9420, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by APC-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
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Irisin/FNDC5 in Rat Skeletal Muscle. Irisin/FNDC5 was detected in perfusion fixed frozen sections of rat skeletal muscle using Mouse Anti-Human Irisin/FNDC5 Monoclonal Antibody (Catalog # MAB9420) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm of muscle cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Irisin/FNDC5
Irisin (also known as FNDC5) is a 12 kDa glycosylated polypeptide hormone that regulates energy metabolism, stem cell differentiation, and neuronal development (1, 2). Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation (2-5). Mature human, mouse, and rat Irisin share 100% sequence identity. Expression of Irisin is induced in skeletal muscle and subcutaneous adipose tissue during and shortly after exercise (2, 6). Irisin induces expression of peroxisome proliferator-activated receptor gamma co-activator 1 alpha (PGC1 alpha ) and uncoupling protein-1 (UCP1), mitochondrial-associated metabolic proteins (7, 8). Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue. In mice, expression of Irisin has been shown to regulate obesity and diabetes (1, 2). A similar function in humans is suggested (9). Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts (10-14).
Product Datasheets
Citation for Human Irisin/FNDC5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Plasma-derived extracellular vesicles released after endurance exercise exert cardioprotective activity through the activation of antioxidant pathways
Authors: Lisi, V;Senesi, G;Bertola, N;Pecoraro, M;Bolis, S;Gualerzi, A;Picciolini, S;Raimondi, A;Fantini, C;Moretti, E;Parisi, A;Sgr�, P;Di Luigi, L;Geiger, R;Ravera, S;Vassalli, G;Caporossi, D;Balbi, C;
Redox biology
Species: Human
Sample Types: Plasma
Applications: Surface Plasmon Resonance (SPR)
FAQs
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