Human IRS2 Antibody Summary
Pro1035-Ile1136
Accession # Q9Y4H2
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human IRS2 by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IRS2 Monoclonal Antibody (Catalog # MAB6347) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IRS2 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IRS2
IRS2 (insulin receptor substrate 2) is a 180-190 kDa substrate intermediate that lies between various cytokine receptors and downstream signaling molecules. It is the primary IRS protein found in hematopoietic cells. Depending upon the receptor, IRS2 undergoes phosphorylation, either via Tyk2 following type I IFN binding, or via JAK1 and 3 following IL-4, -7 and -15 binding. This event allows for its subsequent association with PI-3 kinase. Human IRS2 is 1338 amino acids (aa) in length with a predicted MW of 138 kDa. It contains one PH (pleckstrin homology) domain (aa 16-144), followed by a PTB (phosphotyrosine-binding)domain (aa 194-298) and seven YxxM (Tyr/x/x/Met) motifs (aa 540-1075). IRS2 is phosphorylated on multiple Ser and Tyr residues. There are two potential splice variants. One shows a 16 aa substitution for the C-terminal Glu, while another shows an 11 aa substitution for aa 1314-1338. Over aa 1035-1136, human IRS2 shares 86% aa identity with mouse IRS2.
Product Datasheets
Citations for Human IRS2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
2
Citations: Showing 1 - 2
Filter your results:
Filter by:
-
Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CLpro substrate degradome
Authors: I Pablos, Y Machado, HCR de Jesus, Y Mohamud, R Kappelhoff, C Lindskog, M Vlok, PA Bell, GS Butler, PM Grin, QT Cao, JP Nguyen, N Solis, S Abbina, W Rut, JC Vederas, L Szekely, A Szakos, M Drag, JN Kizhakkeda, K Mossman, JA Hirota, E Jan, H Luo, A Banerjee, CM Overall
Cell Reports, 2021-10-09;37(4):109892.
-
Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CLpro substrate degradome
Authors: I Pablos, Y Machado, HCR de Jesus, Y Mohamud, R Kappelhoff, C Lindskog, M Vlok, PA Bell, GS Butler, PM Grin, QT Cao, JP Nguyen, N Solis, S Abbina, W Rut, JC Vederas, L Szekely, A Szakos, M Drag, JN Kizhakkeda, K Mossman, JA Hirota, E Jan, H Luo, A Banerjee, CM Overall
Cell Reports, 2021-10-09;37(4):109892.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human IRS2 Antibody
There are currently no reviews for this product. Be the first to review Human IRS2 Antibody and earn rewards!
Have you used Human IRS2 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
