Human ISG15/UCRP Antibody
Human ISG15/UCRP Antibody Summary
Accession # P05161
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Immunoprecipitation of Human ISG15/UCRP. A549 cells transfected with HA-ISG15 were treated briefly with IFN gamma or carrier only. 500 µg of cell lysates were subjected to immunoprecipitation (IP) with 20 µg A-830 using Protein G Sepharose. IP’d material was resolved on reducing SDS-PAGE gels and Western Blots were developed using PVDF membranes and anti-HA epitope (R&D Systems MAB060) at 0.5 µg/ml followed by HRP-labeled anti-mouse (R&D Systems # HAF007) secondary antibody at 1:2000 dilution. Free (unconjugated) HA-ISG15 was visualized in both treated and untreated cells, while ISGylated proteins were clearly recovered (IP’d) from the interferon-treated cell lysates.
Preparation and Storage
Background: ISG15/UCRP
Interferonstimulated Gene 15 (ISG15), also known as Ubiquitin Crossreacting Protein (UCRP), is a Ubiquitinlike protein that is covalently coupled to target proteins in a process termed ISGylation. It is a 165 amino acid (aa) polypeptide with a predicted molecular weight of 18 kDa. ISG15/UCRP exhibits 66% aa sequence identity with its mouse ortholog. Structurally, ISG15/UCRP consists of two tandem Ubiquitinlike domains that share a similar 3dimensional structure with Ubiquitin and other Ubiquitinlike modifiers including NEDD8 and SUMO1. Modification of targets by ISG15/UCRP occurs in a stepwise enzymatic process similar to that of Ubiquitin. Enzymes regulating ISGylation include the activating (E1) enzyme UBE1L, the conjugating (E2) enzyme UbcH8, and ligases (E3) such as HERC5. Removal of ISG15/UCRP is catalyzed by the deconjugating enzyme UBP43/USP18. Functionally, ISG15/UCRP has putative roles in the immune response and tumorigenesis. This is reflected by intracellular ISG15/UCRP targets that include Cyclin D1, tumor suppressor p63, IRF3, and a range of viral proteins. It is induced by type 1 interferons and microbial infection, and knockout mice exhibit an increased sensitivity to infection by some viruses. ISG15/UCRP can also be secreted by cells of the immune system and may act in a cytokinelike manner. For instance, it is produced by human granulocytes in response to mycobacterium exposure, and natural killer cells and T cells respond to extracellular ISG15/UCRP with IFNgamma production. Further supporting a role in immune function, ISG15/UCRP mutations are associated with MSMD, an inherited disorder characterized by increased susceptibility to mycobacterial infection.
Product Datasheets
Product Specific Notices
* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.Citations for Human ISG15/UCRP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Deletion of the deISGylating enzyme USP18 enhances tumour cell antigenicity and radiosensitivity
Authors: A Pinto-Fern, M Salio, T Partridge, J Chen, G Vere, H Greenwood, CS Olie, A Damianou, HC Scott, HJ Pegg, A Chiarenza, L Díaz-Saez, P Smith, C Gonzalez-L, B Patel, E Anderton, N Jones, TR Hammonds, K Huber, R Muschel, P Borrow, V Cerundolo, BM Kessler
Br J Cancer, 2020-11-20;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Covalent ISG15 conjugation to CHIP promotes its ubiquitin E3 ligase activity and inhibits lung cancer cell growth in response to type I interferon
Authors: L Yoo, AR Yoon, CO Yun, KC Chung
Cell Death Dis, 2018-01-24;9(2):97.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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