Human ISG15/UCRP Antibody

Product is in the process of being discontinued

Discontinued Product

A-830 has been discontinued.
View all ISG15/UCRP products.
Immunoprecipitation of Human ISG15/UCRP.
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Product Details
Citations (2)
FAQs
Reviews (2)

Human ISG15/UCRP Antibody Summary

Species Reactivity
Human
Specificity
This antibody will recover ISGylated proteins in immunoprecipitation applications. This antibody detects ISGylated proteins in Western blots.
Source
Monoclonal Rat IgG2A Clone # 851701
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Full-length recombinant human ISG15/UCRP
Accession # P05161
Formulation
Supplied as a solution in PBS containing Glycerol and Sodium Azide.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1-0.5 µg/mL
A549 cells transfected with HA-ISG15 treated with IFN-gamma
Immunoprecipitation
Recommended IP conditions: use 10-20 µg of A-830 to recover ISGylated proteins from 0.5 – 1 mg of total protein in 1 ml of cellular lysate. Recommended concentration for Western Blot is 0.1 - 0.5 µg/ml.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunoprecipitation Immunoprecipitation of Human ISG15/UCRP. View Larger

Immunoprecipitation of Human ISG15/UCRP. A549 cells transfected with HA-ISG15 were treated briefly with IFN gamma or carrier only. 500 µg of cell lysates were subjected to immunoprecipitation (IP) with 20 µg A-830 using Protein G Sepharose. IP’d material was resolved on reducing SDS-PAGE gels and Western Blots were developed using PVDF membranes and anti-HA epitope (R&D Systems MAB060) at 0.5 µg/ml followed by HRP-labeled anti-mouse (R&D Systems # HAF007) secondary antibody at 1:2000 dilution. Free (unconjugated) HA-ISG15 was visualized in both treated and untreated cells, while ISGylated proteins were clearly recovered (IP’d) from the interferon-treated cell lysates.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Storage below -20 °C is not recommended. Do not use past expiration date.

Background: ISG15/UCRP

Interferon­stimulated Gene 15 (ISG15), also known as Ubiquitin Cross­reacting Protein (UCRP), is a Ubiquitin­like protein that is covalently coupled to target proteins in a process termed ISGylation. It is a 165 amino acid (aa) polypeptide with a predicted molecular weight of 18 kDa. ISG15/UCRP exhibits 66% aa sequence identity with its mouse ortholog. Structurally, ISG15/UCRP consists of two tandem Ubiquitin­like domains that share a similar 3­dimensional structure with Ubiquitin and other Ubiquitin­like modifiers including NEDD8 and SUMO1. Modification of targets by ISG15/UCRP occurs in a stepwise enzymatic process similar to that of Ubiquitin. Enzymes regulating ISGylation include the activating (E1) enzyme UBE1L, the conjugating (E2) enzyme UbcH8, and ligases (E3) such as HERC5. Removal of ISG15/UCRP is catalyzed by the deconjugating enzyme UBP43/USP18. Functionally, ISG15/UCRP has putative roles in the immune response and tumorigenesis. This is reflected by intracellular ISG15/UCRP targets that include Cyclin D1, tumor suppressor p63, IRF3, and a range of viral proteins. It is induced by type 1 interferons and microbial infection, and knockout mice exhibit an increased sensitivity to infection by some viruses. ISG15/UCRP can also be secreted by cells of the immune system and may act in a cytokine­like manner. For instance, it is produced by human granulocytes in response to mycobacterium exposure, and natural killer cells and T cells respond to extracellular ISG15/UCRP with IFN­gamma production. Further supporting a role in immune function, ISG15/UCRP mutations are associated with MSMD, an inherited disorder characterized by increased susceptibility to mycobacterial infection.

Long Name
ISG15 Ubiquitin-like Modifier
Entrez Gene IDs
9636 (Human); 100038882 (Mouse); 298693 (Rat)
Alternate Names
G1P2; G1P2interferon-induced 17-kDa/15-kDa protein; IFI15; IFI15hUCRP; interferon, alpha-inducible protein (clone IFI-15K); Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; interferon-stimulated protein, 15 kDa; IP17; ISG15 ubiquitin-like modifier; ISG15; Ubiquitin cross-reactive protein; ubiquitin-like protein ISG15; UCRP; UCRPIP17

Product Datasheets

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Product Specific Notices

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

Citations for Human ISG15/UCRP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Deletion of the deISGylating enzyme USP18 enhances tumour cell antigenicity and radiosensitivity
    Authors: A Pinto-Fern, M Salio, T Partridge, J Chen, G Vere, H Greenwood, CS Olie, A Damianou, HC Scott, HJ Pegg, A Chiarenza, L Díaz-Saez, P Smith, C Gonzalez-L, B Patel, E Anderton, N Jones, TR Hammonds, K Huber, R Muschel, P Borrow, V Cerundolo, BM Kessler
    Br J Cancer, 2020-11-20;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  2. Covalent ISG15 conjugation to CHIP promotes its ubiquitin E3 ligase activity and inhibits lung cancer cell growth in response to type I interferon
    Authors: L Yoo, AR Yoon, CO Yun, KC Chung
    Cell Death Dis, 2018-01-24;9(2):97.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human ISG15/UCRP Antibody

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Human ISG15/UCRP Antibody
By Anonymous on 09/30/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Human embryonic kidney 293T cells Species: Human

Human ISG15/UCRP Antibody
By Anonymous on 08/05/2021
Application: WB Sample Tested: HT1080 human fibrosarcoma cell line Species: Human