Human Jak3 Antibody

Name: Human Jak3 Antibody
Brand: R&D Systems
SKU: MAB4699
Rating: 3.5 (2 reviews)

Catalog #: MAB4699 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4699
MAB4699-SP

Detection of Human Jak3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Jak3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Jak3 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 452506

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Jak3
Gly46-Thr209
Accession # P52333

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Jak3 by Western Blot. Western blot shows lysates of TF-1 human erythroleukemic cell line. PVDF membrane was probed with 2 µg/mL of Human Jak3 Monoclonal Antibody (Catalog # MAB4699) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Jak3 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Jak3

Janus Kinase 3 (Jak3) belongs to the Jak family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Also known as Leukocyte Janus Kinase (LJak), Jak3 is activated after binding to the gamma chain of Interleukin receptors, specifically the IL-2 and IL-4 receptors. Activation of Jak3 is associated with the rapid tyrosine phosphorylation of STAT proteins. Defects in Jak3 cause an autosomal severe combined immunodeficiency disease (SCID).

Entrez Gene IDs

3718 (Human); 16453 (Mouse); 25326 (Rat)

Alternate Names

EC 2.7.10; Jak3; JAK3_HUMAN; JAK-3EC 2.7.10.2; JAKL; JAKLtyrosine-protein kinase JAK3; Janus kinase 3Leukocyte janus kinase; LJAK; L-JAKJanus kinase 3 (a protein tyrosine kinase, leukocyte)