Human JAM-A Antibody Summary
Ser28-Ala242
Accession # Q9Y624
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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JAM‑A in MCF‑7 Human Cell Line. JAM-A was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human JAM-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1103) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to intercellular junctions. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: JAM-A
The family of junctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial or epithelial cells. Some family members are also found on blood leukocytes and platelets. Human JAM-A, also known as platelet adhesion molecule 1 (PAM-1) and platelet F11 receptor (3), is predominantly expressed at intercellular junctions of both epithelial cells and endothelial cells (1‑4). It is also expressed on circulating blood cells including neutrophils, monocytes, platelets, erythrocytes and lymphocytes (5). Human JAM-A cDNA predicts a 299 amino acid (aa) residue precursor protein with a putative 27 aa signal peptide, a 210 aa extracellular region containing two Ig‑like V-subset domains, a 24 aa transmembrane domain and a 38 aa cytoplasmic domain. The human and mouse proteins share approximately 67% aa sequence homology. Human JAM-A also shares approximately 35% and 32% aa sequence homology with human JAM-B and JAM-C, respectively. JAM-A exhibits homophilic interactions to regulate tight junction assembly and modulate paracellular permeability. This homophilic interation also mediates platelet aggregation and adhesion to endothelial cells and may play a role in thrombosis (3). JAM-A binds heterotypically with the beta 2 integrin lymphocyte function-associated antigen-1 (LFA-1). This JAM‑A‑LFA‑1 interaction is involved in leukocyte adhesion and transmigration (6). JAM-A has also been shown to bind reovirus attachment protein sigma-1 to permit reovirus infection and signal virus-induced apoptosis (7).
- Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
- Aurand-Lions, M. et al. (2001) Blood 98:3699.
- Sobocka, M.B. et al. (2000) Blood 95:2600.
- Martin-Padura, I. et al. (1998) J. Cell Biol. 142:117.
- Williams, L.A. et. al. (1999) Mol. Immunol. 36:1175.
- Ostermann, G. et al. (2002) Nature Immunol. 3:151.
- Barton, E.S. et al. (2001) Cell 104:441.
Product Datasheets
Citations for Human JAM-A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Helicobacter pylori PqqE is a new virulence factor that cleaves junctional adhesion molecule A and disrupts gastric epithelial integrity
Authors: Miguel S. Marques, Ana C. Costa, Hugo Osório, Marta L. Pinto, Sandra Relvas, Mário Dinis-Ribeiro et al.
Gut Microbes
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miR-143 and miR-145 disrupt the cervical epithelial barrier through dysregulation of cell adhesion, apoptosis and proliferation
Authors: L Anton, A DeVine, LJ Sierra, AG Brown, MA Elovitz
Sci Rep, 2017-06-08;7(1):3020.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
JAM-A and ALCAM are therapeutic targets to inhibit diapedesis across the BBB of CD14+CD16+ monocytes in HIV-infected individuals.
Authors: Williams D, Anastos K, Morgello S, Berman J
J Leukoc Biol, 2014-11-24;97(2):401-12.
Species: Human
Sample Types: Plasma
Applications: ELISA Development (Capture) -
Vascular endothelial tight junctions and barrier function are disrupted by 15(S)-hydroxyeicosatetraenoic acid partly via protein kinase C epsilon-mediated zona occludens-1 phosphorylation at threonine 770/772.
Authors: Chattopadhyay R, Dyukova E, Singh N, Ohba M, Mobley J, Rao G
J Biol Chem, 2013-12-15;289(6):3148-63.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Mesenchymal Stem Cells Transmigrate Between and Directly Through Tumor Necrosis Factor-alpha-Activated Endothelial Cells Via Both Leukocyte-Like and Novel Mechanisms.
Authors: Teo G, Ankrum J, Martinelli R, Boetto S, Simms K, Sciuto T, Dvorak A, Karp J, Carman C
Stem Cells, 2012-11-01;30(11):2472-86.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown.
Authors: Haarmann A, Deiss A, Prochaska J, Foerch C, Weksler B, Romero I, Couraud PO, Stoll G, Rieckmann P, Buttmann M
PLoS ONE, 2010-10-21;5(10):e13568.
Species: Human
Sample Types: Cell Lysates, Serum
Applications: ELISA Development, Western Blot -
Expression, localization, and function of junctional adhesion molecule-C (JAM-C) in human retinal pigment epithelium.
Authors: Economopoulou M, Hammer J, Wang F, Fariss R, Maminishkis A, Miller SS
Invest. Ophthalmol. Vis. Sci., 2008-12-05;50(3):1454-63.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The F11 receptor (F11R/JAM-A) in atherothrombosis: overexpression of F11R in atherosclerotic plaques.
Authors: Babinska A, Azari BM, Salifu MO, Liu R, Jiang XC, Sobocka MB, Boo D, Al Khoury G, Deitch JS, Marmur JD, Ehrlich YH, Kornecki E
Thromb. Haemost., 2007-02-01;97(2):272-81.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Junctional adhesion molecule 1 is a functional receptor for feline calicivirus.
Authors: Makino A, Shimojima M, Miyazawa T, Kato K, Tohya Y, Akashi H
J. Virol., 2006-05-01;80(9):4482-90.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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