Human JAM-C Antibody

Catalog #: AF1189
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1189
AF1189-SP

Detection of Human JAM‑C by Western Blot.

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All JAM-C Antibodies

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Citations (6)

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Human JAM-C Antibody Summary

Species Reactivity

Human

Specificity

Detects human JAM‑C in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 75% cross-reactivity with recombinant mouse (rm) JAM-C is observed and less than 1% cross-reactivity with recombinant human JAM-1 and rmJAM-1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human JAM-C
Val32-Asn241 (Ala149Pro)
Accession # Q9BX67

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

20 µg/mL

See below

Flow Cytometry

2.5 µg/10⁶ cells

Human platelets

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Neutralization

Measured by its ability to neutralize JAM‑C-mediated adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line. Fong, S. et al. (2002) J. Immunol. 168:1618. The Neutralization Dose (ND₅₀) is typically 2‑8 µg/mL in the presence of 0.2 µg/mL Recombinant Human JAM‑B Fc Chimera.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human JAM‑C by Western Blot. Western blot shows lysates of human placenta tissue, human brain tissue, JAR human choriocarcinoma cell line, and JEG‑3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human JAM‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1189) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for JAM‑C at approximately 36-38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human JAM‑C by Simple Western^TM. Simple Western lane view shows lysates of JAR human choriocarcinoma cell line and human placenta, loaded at 0.2 mg/mL. A specific band was detected for JAM-C at approximately 53 and 54 kDa (as indicated) using 20 µg/mL of Goat Anti-Human JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1189) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Neutralization

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Cell Adhesion Mediated by JAM‑C and Neutralization by Human JAM‑C Antibody. Recombinant Human JAM-B/ VE-JAM Fc Chimera (Catalog # 1074-VJ), immobilized onto a microplate previously coated with Goat Anti-Human IgG Fc (Catalog # G-102-C), supports the adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Human JAM-B Fc Chimera (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Human JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1189). The ND₅₀ is typically 2-8 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JAM-C

The family of juctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. Human JAM-C cDNA predicts a 310 amino acid (aa) residue precursor protein with a putative 31 aa signal peptide, a 210 aa extracellular region containing two Ig domains, a 23 aa transmembrane domain and a 46 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site (3, 4). Human JAM-C shares 86% aa sequence identity with its mouse homologue. It also shares approximately 36% and 32% aa sequence homology with human JAM-B and JAM-A, respectively (3‑5). Human JAM-C shows widespread tissue expression and the highest levels are found in the placenta, brain, kidney and heart. JAM-C is expressed on endothelial cells of high endothelial venules in human tonsil. It is also expressed on platelets, T-cells and NK cells (3‑5). Unlike other JAM family members, JAM-C forms only weak homotypic interactions. JAM-C binds to JAM-B to facilitate the interactions between JAM-B and the integrin alpha4beta1 (6). This heterotypic interaction between leukocyte JAM-C and endothelial JAM-B may play a role in regulating leukocyte transmigration (5). On platelets, JAM-C is a counter-receptor for the leukocyte integrin Mac-1(CD11b/CD18) (7). JAM-C has also been identified as a strong candidate gene for hypoplastic left heart syndrome (8).

The nomenclature used for the JAM family proteins is confusing. VE-JAM has been referred to in the literature variously as JAM-B or JAM-C. Until further clarification, R&D Systems has adopted the nomenclature where both mouse and human VE-JAM are referred to as JAM-B. Under this system, JAM-C refers to the protein encoded by the gene localized to human chromosome 11.

References

Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
Aurand-Lions, M. et al. (2001) Blood 98:3699.
Arrate, M.P. et al. (2001) J. Biol. Chem. 276:45826.
Liang, T. et al. (2002) J. Immunol. 168:1618.
Johnson-Leger, C. et al. (2002) Blood 100:25793.
Cunningham, A. et al. (2002) J Biol. Chem. 277:27589.
Santoso, S. et al. (2002) J. Exp. Med. 196:679.
Phillips, H.M. et al. (2002) Genomics 79:475.

Long Name

Junctional Adhesion Molecule C

Entrez Gene IDs

83700 (Human); 83964 (Mouse)

Alternate Names

CD323; JAM-2; JAM3; JAMC; JAM-C; JAM-CFLJ14529; junctional adhesion molecule 3JAM-3; junctional adhesion molecule C

Product Datasheets

Product Datasheet

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Citations for Human JAM-C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Deficiency of Jamc Leads to Congenital Nuclear Cataract and Activates the Unfolded Protein Response in Mouse Lenses
Authors: J Li, X Tan, Q Sun, X Li, R Chen, L Luo
Investigative Ophthalmology & Visual Science, 2022-09-01;63(10):1.
Species: Mouse
Sample Types: Tissue Lysate
Applications: Western Blot
Aberrant stromal tissue factor localisation and mycolactone-driven vascular dysfunction, exacerbated by IL-1beta, are linked to fibrin formation in Buruli ulcer lesions
Authors: LT Hsieh, SJ Dos Santos, BS Hall, J Ogbechi, AD Loglo, FJ Salguero, MT Ruf, G Pluschke, RE Simmonds
PloS Pathogens, 2022-01-31;18(1):e1010280.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Junctional adhesion molecule (JAM)-B supports lymphocyte rolling and adhesion through interaction with alpha4beta1 integrin.
Authors: Ludwig RJ, Hardt K, Hatting M, Bistrian R, Diehl S, Radeke HH, Podda M, Schon MP, Kaufmann R, Henschler R, Pfeilschifter JM, Santoso S, Boehncke WH
Immunology, 2009-10-01;128(2):196-205.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Junctional adhesion molecule-C promotes metastatic potential of HT1080 human fibrosarcoma.
Authors: Fuse C, Ishida Y, Hikita T, Asai T, Oku N
J. Biol. Chem., 2007-01-16;282(11):8276-83.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
JAM-C is a component of desmosomes and a ligand for CD11b/CD18-mediated neutrophil transepithelial migration.
Authors: Zen K, Babbin BA, Liu Y, Whelan JB, Nusrat A, Parkos CA
Mol. Biol. Cell, 2004-06-11;15(8):3926-37.
Species: Human
Sample Types: Cell Lysates, Recombinant Protein, Whole Cells
Applications: ELISA Development, ICC, Western Blot
Neutrophil‐endothelial interactions of murine cells is not a good predictor of their interactions in human cells
Authors: Fariborz Soroush, Yuan Tang, Omar Mustafa, Shuang Sun, Qingliang Yang, Laurie E. Kilpatrick et al.
The FASEB Journal