Human Jumonji/JARID2 Antibody

Catalog #: MAB6090 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6090
MAB6090-SP

Jumonji/JARID2 in LNCaP Human Cell Line.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Jumonji/JARID2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Jumonji/JARID2 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 674702

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Jumonji/JARID2
Met1-Thr159
Accession # Q92833

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Jumonji/JARID2 in LNCaP Human Cell Line. Jumonji/ JARID2 was detected in immersion fixed LNCaP human prostate cancer cell line using Mouse Anti-Human Jumonji/JARID2 Monoclonal Antibody (Catalog # MAB6090) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Jumonji/JARID2

JARID2 (Jumonji/ARID domain-containing protein 2; also Jumonji [Japanese for “cruciform/cross”] and JMJ) is a 150-160 kDa member of the JMJ family of proteins. It is a nuclear complex coordinator that interacts with the G9a and GLP methyltransferases to repress gene transcription. JARID2 is expressed in mammalian embryonic neurons, megakaryocytes, hepatocytes and cardiomyocytes. Human JARID2 is 1246 amino acids (aa) in length. It contains an NLS (aa 1-131), a transcriptional repressor domain (aa 132-222), a DNA binding region (aa 558-709) and a nonfunctional histone demethylase domain (aa 916-1042). There are potential splice variants. One shows an alternative start site at Met173, another shows a deletion of aa 304-1151, and a third contains a 22 aa substitution for aa 1-60, coupled to a 50 aa substitution for aa 949-1246. Over aa 1-159, human JARID2 shares 96% aa identity with mouse JARID2.

Entrez Gene IDs

3720 (Human); 16468 (Mouse)

Alternate Names

JARID2; JMJ; JMJjumonji-like protein; jumonji (mouse) homolog; jumonji homolog; Jumonji; jumonji, AT rich interactive domain 2; Jumonji/ARID domain-containing protein 2; protein Jumonji