Human JunB Antibody

Catalog #: AF4456 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4456
AF4456-SP

Detection of Human JunB by Western Blot.

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Human JunB Antibody Summary

Species Reactivity

Human

Specificity

Detects human JunB in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human JunB

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human JunB by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human JunB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4456) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for JunB was detected at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JunB

JunB is a member of the bzip Jun family of transcription factors. JunB binds as a homo or heterodimer, with other members of the Jun/Fos family, to the DNA element TGA[CG]TCA. Knock out mice indicate that JunB is involved in erythroid differentiation and hematopoietic stem cell viability.

Long Name

JunB Proto-oncogene

Entrez Gene IDs

3726 (Human); 16477 (Mouse); 24517 (Rat)

Alternate Names

activator protein 1; AP-1; jun B proto-oncogene; JunB; transcription factor jun-B