Human KA1/GRIK4 Antibody

Catalog #: MAB113261 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB113261-100
MAB113261-SP

Detection of KA1/GRIK4 in Human Brain Cortex.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human KA1/GRIK4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human KA1/GRIK in direct ELISA.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2915C

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human KA1/GRIK containing peptide
Accession # P42263

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of Human Brain Cortex

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of KA1/GRIK4 in Human Brain Cortex. KA1/GRIK4 was detected in immersion fixed paraffin-embedded sections of Human Brain Cortex using Rabbit Anti-Human KA1/GRIK4 Monoclonal Antibody (Catalog # MAB113261) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glial cells and in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KA1/GRIK4

GRIK4 (Glutamate receptor, ionotropic kainate 4) is a receptor for glutamate and is one of the 5 types of kainate receptor subunits. Glutamate acts as an excitatory neurotransmitter at many synapses in the CNS are key mediators of brain communication. Kainate receptors are relevant targets for neuroprotection of both neurons and glia in acute and chronic neurodegerative diseases. They are also involved in epilepsy, pain and psychiatric disorders. Variations in GRIK4 have been associated with both increased and decreased risk of bipolar disorder. Single-nucleotide polymorphism's in GRIK 4 are also observed in Schizophrenia.

References

Entrez Gene: GRIK4 glutamate receptor, ionotropic, kainate 4.
Szpirer C, Molne M, Antonacci R, Jenkins NA, Finelli P, Szpirer J, Riviere M, Gilbert DJ, Copeland NG, et al. "The Genes Encoding the Glutamate Receptor Subunits Ka1 and KA2 (GRIK4 and GRIK5) are located on separate chromosomes in Human, Mouse and Rat". Proc Natl Acad Sci U.S.A. 1995 Jan; 91(25):11849-53.
Contractor A, Mulle C, Swanson GT. "Kainate Receptors Coming of Age: Milestones of Two Decades of Research". Trends in Neurosciences. 2011 Mar; 34(3):154.
Matute C. "Therapeutic Potential of Kainate Receptors". CNS Neuroscience & Therapeutics. 2011 Dec; 17(6):661.

Long Name

Glutamate Receptor KA1

Entrez Gene IDs

2900 (Human); 110637 (Mouse); 24406 (Rat)

Alternate Names

EAA1; Excitatory amino acid receptor 1; GluK4; glutamate receptor KA1; Glutamate receptor KA-1; glutamate receptor, ionotropic kainate 4; glutamate receptor, ionotropic, kainate 4; GRIK4; KA1; KA1GRIK