Human KCC4/SLC12A7 Antibody

Catalog #: MAB9030
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9030
MAB9030-SP

Detection of KCC4/SLC12A7 in HEK293 Human Cell Line Transfected with Human SLC12A7 and eGFP by Flow Cytometry.

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All KCC4/SLC12A7 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human KCC4/SLC12A7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SLC12A7 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 891526

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human SLC12A7
Met1-Ser1083
Accession # Q9Y666

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of KCC4/SLC12A7 in HEK293 Human Cell Line Transfected with Human SLC12A7 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human SLC12A7 or (B) human MRGPRF and eGFP was stained with Mouse Anti-Human KCC4/SLC12A7 Monoclonal Antibody (Catalog # MAB9030) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KCC4/SLC12A7

Cation chloride cotransporters, including the potassium-chloride cotransporters (KCCs), are involved in the electroneutral movement of ions across the plasma membrane. SLC12A7 contains 12 membrane-spanning segments, 8 phosphorylation sites, 7 of which are in the C terminus, and 4 potential N-glycosylation sites. It is important for potassium recycling by siphoning potassium ions after their exit from outer hair cells into supporting Deiters cells, where potassium enters the gap junction pathway.

Long Name

Potassium/Chloride Transporter KCC4

Entrez Gene IDs

10723 (Human); 20499 (Mouse); 308069 (Rat)

Alternate Names

DKFZp434F076; KCC4; KCC4Electroneutral potassium-chloride cotransporter 4; K-Cl cotransporter 4; potassium/chloride transporter KCC4; SLC12A7; solute carrier family 12 (potassium/chloride transporters), member 7; solute carrier family 12 member 7

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human KCC4/SLC12A7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1