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Human KGF/FGF-7 Antibody

Catalog #: AF-251-NA
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-251-NA
AF-251-SP
Cell Proliferation Induced by KGF/FGF‑7 and Neutralization by Human KGF/FGF‑7 Antibody.
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Citations (10)
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Human KGF/FGF-7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human KGF/FGF‑7 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse FGF-7 is observed, less than 20% cross-reactivity with recombinant human (rh) FGF-9 is observed, and less than 10% cross‑reactivity with rhFGF-4 and rhFGF-9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human KGF/FGF‑7
Cys32-Thr194
Accession # Q6FGV5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human KGF/FGF‑7 (Catalog # 251-KG)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize KGF/FGF‑7-induced proliferation in the 4MBr‑5 rhesus monkey epithelial cell line. The Neutralization Dose (ND50) is typically 6-12 µg/mL in the presence of 125 ng/mL Recombinant Human KGF/FGF‑7.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by KGF/FGF‑7 and Neutralization by Human KGF/FGF‑7 Antibody. View Larger

Cell Proliferation Induced by KGF/FGF‑7 and Neutralization by Human KGF/FGF‑7 Antibody. Recombinant Human KGF/FGF‑7 (Catalog # 251-KG) stimulates proliferation in the 4MBr‑5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human KGF/FGF‑7 (125 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human KGF/FGF‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-251-NA). The ND50 is typically 6-12 µg/mL.

Immunohistochemistry KGF/FGF-7 antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

KGF/FGF‑7 in Human Placenta. KGF/FGF-7 was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using Goat Anti-Human KGF/FGF-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-251-NA) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to trophoblast cells in chorionic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry-Paraffin Detection of Porcine KGF/FGF-7 by Immunohistochemistry-Paraffin View Larger

Detection of Porcine KGF/FGF-7 by Immunohistochemistry-Paraffin Immunohistochemical detection of KGF-2 in biopsies of DNCB-induced rashes treated with various nanocrystalline silver-derived solutions. (A) Representative images are shown for immunohistochemical detection of KGF-2 after 24 h treatment of negative controls with distilled water (i), and DNCB-induced porcine contact dermatitis rashes with distilled water (positive controls) (ii), or nanocrystalline silver-derived solutions generated at starting pHs of 4 (iii), 5.6 (iv), 7 (v), or 9 (vi). The scale bar in A represents 100 μm. Staining for KGF-2 appears brown, while the cell nuclei are counterstained purple using haematoxylin. Immunohistochemical staining scores for KGF-2 are shown after 24 h (B) and 72 h (C) of treatment as described above. Statistical analyses, which were performed using one-way ANOVAs with Tukey-Kramer Multiple Comparisons post tests, are shown in Table 4. Error bars represent standard deviations. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/20170497), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry-Paraffin Detection of Porcine KGF/FGF-7 by Immunohistochemistry-Paraffin View Larger

Detection of Porcine KGF/FGF-7 by Immunohistochemistry-Paraffin Immunohistochemical detection of KGF-2 in biopsies of DNCB-induced rashes treated with various nanocrystalline silver-derived solutions. (A) Representative images are shown for immunohistochemical detection of KGF-2 after 24 h treatment of negative controls with distilled water (i), and DNCB-induced porcine contact dermatitis rashes with distilled water (positive controls) (ii), or nanocrystalline silver-derived solutions generated at starting pHs of 4 (iii), 5.6 (iv), 7 (v), or 9 (vi). The scale bar in A represents 100 μm. Staining for KGF-2 appears brown, while the cell nuclei are counterstained purple using haematoxylin. Immunohistochemical staining scores for KGF-2 are shown after 24 h (B) and 72 h (C) of treatment as described above. Statistical analyses, which were performed using one-way ANOVAs with Tukey-Kramer Multiple Comparisons post tests, are shown in Table 4. Error bars represent standard deviations. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/20170497), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KGF/FGF-7

KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1‑4). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha. (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature human KGF, which is active across species, shares 98% aa sequence identity with bovine, equine, ovine and canine, 96% with mouse and porcine, and 92% with rat KGF, respectively.

References
  1. Finch, P.W. and J.S. Rubin (2006) J. Natl. Cancer Inst. 98:812.
  2. Werner, S. et al. (2007) J. Invest. Dermatol. 127:998.
  3. Werner, S. (1998) Cytokine Growth Factor Rev. 9:153.
  4. Mason, I.J. et al. (1994) Mech. Dev. 45:15.
  5. Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
  6. Niu, J. et al. (2007) J. Biol. Chem. 282:6001.
  7. Cardinali, G. et al. (2005) J. Invest. Dermatol. 125:1190.
  8. Qiao, J. et al. (1999) Development 126:547.
  9. Guo, L. et al. (1996) Genes Dev. 10:165.
  10. de Georgi, V. et al. (2007) Dermatol. Clin. 25:477.
  11. Hsu, Y-R. et al. (1999) Biochemistry 38:2523.
  12. Belleudi, F. et al. (2007) Traffic 8:1854.
Long Name
Keratinocyte Growth Factor
Entrez Gene IDs
2252 (Human); 403915 (Canine)
Alternate Names
FGF7; FGF-7; fibroblast growth factor 7HBGF-7; HBGF7; HBGF-7; Heparin-binding growth factor 7; keratinocyte growth factor; KGF; KGFfibroblast growth factor 7 (keratinocyte growth factor)

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Citations for Human KGF/FGF-7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Three subtypes of lung cancer fibroblasts define distinct therapeutic paradigms.
    Authors: Haichuan H, Zofia P, Patricia H et al.
    Cancer Cell.
  2. PLAC1 is essential for FGF7/FGFRIIIb-induced Akt-mediated cancer cell proliferation
    Authors: DB Roldán, M Grimmler, C Hartmann, S Hubich-Rau, T Bei beta ert, C Paret, G Cagna, C Rohde, S Wöll, M Koslowski, Ö Türeci, U Sahin
    Oncotarget, 2020-05-19;11(20):1862-1875.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: Co-Immunoprecipitation, IHC
  3. Human Multilineage-differentiating Stress-Enduring Cells Exert Pleiotropic Effects to Ameliorate Acute Lung Ischemia-Reperfusion Injury in a Rat Model
    Authors: H Yabuki, S Wakao, Y Kushida, M Dezawa, Y Okada
    Cell Transplant, 2018-04-30;0(0):9636897187616.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Sestrin-2, a repressor of PDGFR beta signalling, promotes cigarette-smoke-induced pulmonary emphysema in mice and is upregulated in individuals with COPD
    Authors: Juliana Heidler, Athanasios Fysikopoulos, Frank Wempe, Michael Seimetz, Thorsten Bangsow, Ana Tomasovic et al.
    Disease Models & Mechanisms
  5. Expression and Function of Fibroblast Growth Factor (FGF) 7 during Liver Regeneration
    Authors: Su-Mei Tsai, Wen-Pin Wang
    Cellular Physiology and Biochemistry
  6. Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
    Authors: Nadworny PL, Wang J, Tredget EE
    J Inflamm (Lond), 2010-02-19;7(0):13.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
    Authors: Skibinski G, Elborn JS, Ennis M
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007-03-23;293(1):L69-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells.
    Authors: Lee JH, Ryu IH, Kim EK, Lee JE, Hong S, Lee HK
    Invest. Ophthalmol. Vis. Sci., 2006-03-01;47(3):864-72.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Salivary leptin induces increased expression of growth factors in oral keratinocytes.
    Authors: Groschl M, Topf HG, Kratzsch J, Dotsch J, Rascher W, Rauh M
    J. Mol. Endocrinol., 2005-04-01;34(2):353-66.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Coculture with bone marrow‑derived mesenchymal stem cells attenuates inflammation and apoptosis in lipopolysaccharide‑stimulated alveolar epithelial cells via enhanced secretion of keratinocyte growth factor and angiopoietin‑1 modulating the Toll‑like receptor‑4 signal pathway
    Authors: Xu‑Xin Chen, Lu Tang, Zhi‑Hai Han, Wen‑Jing Wang, Ji‑Guang Meng
    Molecular Medicine Reports

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