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Human KIR/CD158 PE-conjugated Antibody

Catalog #: FAB1848P
5 Citations Datasheet / COA / SDS
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FAB1848P
Detection of KIR/CD158 in Human PBMCs by Flow Cytometry.
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Citations (5)
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Human KIR/CD158 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human KIR/CD158. Stains cells transfected with KIR2DL2, 2DL3, 2DS2, or 2DS4. It does not stain cells transfected with KIR2DL1, 2DL4, 3DL1, or 3DL2.
Source
Monoclonal Mouse IgG2B Clone # 180704
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human KIR2DL3
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of KIR/CD158 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of KIR/CD158 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human KIR/CD158 PE-conjugated Monoclonal Antibody (Catalog # FAB1848P) and Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KIR/CD158

KIR is a family of immunoglobulin-like receptors expressed on NK cells and some T cells. Family members with long (L) cytoplasmic tails deliver inhibitory signals and those with short (S) cytoplasmic tails deliver activating signals. Several KIR proteins are known to recognize particular alleles of MHC class I proteins.

Alternate Names
CD158 antigen-like family member A; CD158; CD158a antigen; CD158Ankat1; cl-42; killer cell immunoglobulin-like receptor 2DL1; killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 1; killer Ig receptor; killer inhibitory receptor 2-2-1; KIR; KIR221; MHC class I NK cell receptor; Natural killer-associated transcript 1; NKAT; NKAT-1; NKAT1KIR-K64; p58 killer cell inhibitory receptor KIR-K64; p58 natural killer cell receptor clones CL-42/47.11; p58 NK cell inhibitory receptor NKR-K6,47.11; p58 NK receptor CL-42/47.11; p58.1 MHC class-I-specific NK receptor; p58.1

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Citations for Human KIR/CD158 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Quadruple gene-engineered natural killer cells enable multi-antigen targeting for durable antitumor activity against multiple myeloma
    Authors: F Cichocki, R Bjordahl, JP Goodridge, S Mahmood, S Gaidarova, R Abujarour, ZB Davis, A Merino, K Tuininga, H Wang, A Kumar, B Groff, A Witty, G Bonello, J Huffman, T Dailey, TT Lee, KJ Malmberg, B Walcheck, U Höpken, A Rehm, B Valamehr, JS Miller
    Nature Communications, 2022-11-29;13(1):7341.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Identification of human NK17/NK1 cells.
    Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
    PLoS ONE, 2011-10-21;6(10):e26780.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Natural killer lysis receptor (NKLR)/NKLR-ligand matching as a novel approach for enhancing anti-tumor activity of allogeneic NK cells.
    Authors: Markel G, Seidman R, Besser MJ
    PLoS ONE, 2009-05-19;4(5):e5597.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Human leukocyte antigen-G, a ligand for the natural killer receptor KIR2DL4, is expressed by eutopic endometrium only in the menstrual phase.
    Authors: Kawashima M, Maeda N, Adachi Y, Takeuchi T, Yamamoto Y, Izumiya C, Hayashi K, Furihata M, Udaka K, Fukaya T
    Fertil. Steril., 2008-03-07;91(2):343-9.
    Species: Human
    Sample Types: Peritoneal Fluid
    Applications: Flow Cytometry
  5. Histone deacetylase inhibitors suppress natural killer cell cytolytic activity.
    Authors: Ogbomo H, Michaelis M, Kreuter J, Doerr HW, Cinatl J
    FEBS Lett., 2007-03-01;581(7):1317-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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