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Human KIR2DL1/KIR2DS5 PerCP-conjugated Antibody

Catalog #: FAB1844C
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1844C-025
FAB1844C-100
Detection of KIR2DL1/KIR2DS5 in Human PBMCs by Flow Cytometry.
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Human KIR2DL1/KIR2DS5 PerCP-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human KIR2DL1/KIR2DS5 on transfected cells. It does not stain cells transfected with KIR2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 2DS4, 3DL1, 3DL2, or 3DS1.
Source
Monoclonal Mouse IgG1 Clone # 143211
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human KIR2DL1/KIR2DS5
Accession # P43626
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of KIR2DL1/KIR2DS5 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of KIR2DL1/KIR2DS5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human KIR2DL1/KIR2DS5 PerCP-conjugated Monoclonal Antibody (Catalog # FAB1844C) and Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A). Quadrant markers were set based on control antibody staining (Catalog # IC002C). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KIR2DL1/KIR2DS5

KIR2DL1 is one of several immunoglobulin-like receptors expressed on NK cells that bind MHC class I molecules and transmit inhibitory signals. KIR2DL1 is specific for HLA-C alleles with Asn77 and Lys80.

Alternate Names
CD158a; KIR2DL1/KIR2DS5

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Citation for Human KIR2DL1/KIR2DS5 PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Selective Expansion of NKG2C+ Adaptive NK Cells Using K562 Cells Expressing HLA-E
    Authors: MT Phan, J Kim, SK Koh, Y Lim, H Yu, M Lee, JM Lee, ES Kang, HY Kim, SK Kim, I Hwang, D Cho
    International Journal of Molecular Sciences, 2022-08-20;23(16):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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