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Human KIR2DL3/CD158b2 Antibody

Catalog #: MAB2014
12 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2014
MAB2014-SP
Detection of KIR2DL3/CD158b2 in Human PBMCs by Flow Cytometry.
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Citations (12)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human KIR2DL3/CD158b2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human KIR2DL3/CD158b2. Stains BaF3 cells transfected with human KIR2DL3 in flow cytometry. Does not stain BaF3 cells transfected with KIR2DL1, 2DL2, 2DL4, 2DL5, 2DS1, 2DS2, 2DS4, 3DL1, 3DL2, or 3DS1.
Source
Monoclonal Mouse IgG2A Clone # 180701
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human KIR2DL3/CD158b2
Accession # P43628
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-reported
Horowitz, A. et al. (2015) J. Immunol. 195: 4524. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of KIR2DL3/CD158b2 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of KIR2DL3/CD158b2 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human KIR2DL3/CD158b2 Monoclonal Antibody (Catalog # MAB2014) or (B) Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P).View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KIR2DL3/CD158b2

KIR2DL3 is one of several immunoglobulin-like receptors expressed on NK and T cells that bind MHC class I molecules and regulate killer cell activity. KIR2DL3 interacts specifically with HLA-Cw3.

Long Name
Killer Cell Immunoglobulin-like Receptor, Two Domain Long Cytoplasmic Tail, 3
Entrez Gene IDs
3804 (Human)
Alternate Names
CD158 antigen-like family member B2; CD158b2 antigen; CD158b2; CD158B2CD158b; cl-6; killer cell immunoglobulin-like receptor 2DL3; killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 3; Killer inhibitory receptor cl 2-3; KIR-023GB; KIR2DL3; KIRCL23; KIR-K7b; KIR-K7c; MGC129943; MHC class I NK cell receptor; natural killer associated transcript 2; natural killer cell inhibitory receptor KIR2DL3; Natural killer-associated transcript 2; NKAT; NKAT2; NKAT-2; NKAT2A; nkat2b; NKAT2GL183; NK-receptor; p58 natural killer cell receptor clone CL-6; p58 NK receptor CL-6; p58; p58.2 MHC class-I specific NK receptor; p58.2 MHC class-I-specific NK receptor

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Citations for Human KIR2DL3/CD158b2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. LILRB1-HLA-G axis defines a checkpoint driving natural killer cell exhaustion in tuberculosis
    Authors: Wang, J;Chai, Q;Lei, Z;Wang, Y;He, J;Ge, P;Lu, Z;Qiang, L;Zhao, D;Yu, S;Qiu, C;Zhong, Y;Li, BX;Zhang, L;Pang, Y;Gao, GF;Liu, CH;
    EMBO molecular medicine
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTOF-reported
  2. Centromeric KIR AA Individuals Harbor Particular KIR Alleles Conferring Beneficial NK Cell Features with Implications in Haplo-Identical Hematopoietic Stem Cell Transplantation
    Authors: L Dubreuil, B Maniangou, P Chevallier, A Quéméner, N Legrand, MC Béné, C Willem, G David, M Alizadeh, DR Makanga, A Cesbron, K Gendzekhad, K Gagne, C Retière
    Cancers, 2020-12-01;12(12):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. KIR+ CD8+ T Lymphocytes in Cancer Immunosurveillance and Patient Survival: Gene Expression Profiling
    Authors: L Gimeno, EM Serrano-Ló, JA Campillo, MA Cánovas-Za, OS Acuña, F García-Cóz, MV Martínez-S, MD Martínez-H, MF Soto-Ramír, P López-Cubi, J Martínez-E, J Martínez-G, S Corbalan-G, MR Álvarez-Ló, A Minguela
    Cancers (Basel), 2020-10-15;12(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Missing self triggers NK cell-mediated chronic vascular rejection of solid organ transplants
    Authors: A Koenig, CC Chen, A Marçais, T Barba, V Mathias, A Sicard, M Rabeyrin, M Racapé, JP Duong-Van-, P Bruneval, A Loupy, S Dussurgey, S Ducreux, V Meas-Yedid, JC Olivo-Mari, H Paidassi, R Guillemain, JL Taupin, J Callemeyn, E Morelon, A Nicoletti, B Charreau, V Dubois, M Naesens, T Walzer, T Defrance, O Thaunat
    Nat Commun, 2019-11-25;10(1):5350.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. HIV-1 Control by NK Cells via Reduced Interaction between KIR2DL2 and HLA-C(?)12:02/C(?)14:03
    Cell Rep, 2016-11-22;17(9):2210-2220.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. NK Cell Responses to Human Tick-Borne Encephalitis Virus Infection
    J Immunol, 2016-08-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Regulation of Adaptive NK Cells and CD8 T Cells by HLA-C Correlates with Allogeneic Hematopoietic Cell Transplantation and with Cytomegalovirus Reactivation.
    Authors: Horowitz A, Guethlein L, Nemat-Gorgani N, Norman P, Cooley S, Miller J, Parham P
    J Immunol, 2015-09-28;195(9):4524-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof
  8. Tissue-Specific Education of Decidual NK Cells.
    Authors: Sharkey A, Xiong S, Kennedy P, Gardner L, Farrell L, Chazara O, Ivarsson M, Hiby S, Colucci F, Moffett A
    J Immunol, 2015-08-28;195(7):3026-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Coordinated expression of DNAM-1 and LFA-1 in educated NK cells.
    Authors: Enqvist M, Ask E, Forslund E, Carlsten M, Abrahamsen G, Beziat V, Andersson S, Schaffer M, Spurkland A, Bryceson Y, Onfelt B, Malmberg K
    J Immunol, 2015-03-30;194(9):4518-27.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Licensed human natural killer cells aid dendritic cell maturation via TNFSF14/LIGHT.
    Authors: Holmes T, Wilson E, Black E, Benest A, Vaz C, Tan B, Tanavde V, Cook G
    Proc Natl Acad Sci U S A, 2014-12-15;111(52):E5688-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  11. Modulation of the natural killer cell KIR repertoire by cytomegalovirus infection.
    Authors: Charoudeh H, Terszowski G, Czaja K, Gonzalez A, Schmitter K, Stern M
    Eur J Immunol, 2012-12-12;43(2):480-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. NK cell terminal differentiation: correlated stepwise decrease of NKG2A and acquisition of KIRs.
    Authors: Beziat V, Descours B, Parizot C, Debre P, Vieillard V
    PLoS ONE, 2010-08-06;5(8):e11966.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human KIR2DL3/CD158b2 Antibody
By Anonymous on 06/15/2018
Application: Flow Sample Tested: HT1080 human fibrosarcoma cell line Species: Human