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Human KIR2DL3/CD158b2 Fluorescein-conjugated Antibody

Catalog #: FAB2014F
12 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB2014F-025
FAB2014F-100
Detection of KIR2DL3/CD158b2 in Human PBMCs by Flow Cytometry.
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Citations (12)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human KIR2DL3/CD158b2 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human KIR2DL3/CD158b2. Stains BaF3 cells transfected with human KIR2DL3 in flow cytometry. Does not stain BaF3 cells transfected with KIR2DL1, 2DL2, 2DL4, 2DL5, 2DS1, 2DS2, 2DS4, 3DL1, 3DL2, or 3DS1.
Source
Monoclonal Mouse IgG2A Clone # 180701
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human KIR2DL3/CD158b2
Accession # P43628
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of KIR2DL3/CD158b2 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of KIR2DL3/CD158b2 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human KIR2DL3/CD158b2 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2014F) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KIR2DL3/CD158b2

KIR2DL3 is one of several immunoglobulin-like receptors expressed on NK and T cells that bind MHC class I molecules and regulate killer cell activity. KIR2DL3 interacts specifically with HLA-Cw3.

Long Name
Killer Cell Immunoglobulin-like Receptor, Two Domain Long Cytoplasmic Tail, 3
Entrez Gene IDs
3804 (Human)
Alternate Names
CD158 antigen-like family member B2; CD158b2 antigen; CD158b2; CD158B2CD158b; cl-6; killer cell immunoglobulin-like receptor 2DL3; killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 3; Killer inhibitory receptor cl 2-3; KIR-023GB; KIR2DL3; KIRCL23; KIR-K7b; KIR-K7c; MGC129943; MHC class I NK cell receptor; natural killer associated transcript 2; natural killer cell inhibitory receptor KIR2DL3; Natural killer-associated transcript 2; NKAT; NKAT2; NKAT-2; NKAT2A; nkat2b; NKAT2GL183; NK-receptor; p58 natural killer cell receptor clone CL-6; p58 NK receptor CL-6; p58; p58.2 MHC class-I specific NK receptor; p58.2 MHC class-I-specific NK receptor

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Citations for Human KIR2DL3/CD158b2 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Umbilical cord blood-derived ILC1-like cells constitute a novel precursor for mature KIR+NKG2A- NK cells
    Authors: Sabrina Bianca Bennstein, Sandra Weinhold, Angela Riccarda Manser, Nadine Scherenschlich, Angela Noll, Katharina Raba et al.
    eLife
  2. Phenotypic and Functional Changes in Peripheral Blood Natural Killer Cells in Crohn Disease Patients.
    Authors: Samarani S, Sagala P, Jantchou P, Grimard G, Faure C, Deslandres C, Amre D, Ahmad A
    Mediators Inflamm, 2020-02-19;2020(0):6401969.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Intra-lineage Plasticity and Functional Reprogramming Maintain Natural Killer Cell Repertoire Diversity
    Authors: A Pfefferle, B Jacobs, H Netskar, EH Ask, S Lorenz, T Clancy, JP Goodridge, E Sohlberg, KJ Malmberg
    Cell Rep, 2019-11-19;29(8):2284-2294.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Endometrial natural killer (NK) cells reveal a tissue-specific receptor repertoire
    Authors: D Feyaerts, T Kuret, B van Cranen, S van der Ze, OWH van der He, A van der Me, I Joosten, RG van der Mo
    Hum. Reprod., 2018-03-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Complete Remission with Reduction of High-risk Clones following Haploidentical NK Cell Therapy against MDS and AML
    Authors: AT Björklund, M Carlsten, E Sohlberg, LL Liu, T Clancy, M Karimi, S Cooley, JS Miller, M Klimkowska, M Schaffer, E Watz, KI Wikstrom, P Blomberg, BE Wahlin, M Palma, L Hansson, P Ljungman, E Hellström-, HG Ljunggren, KJ Malmberg
    Clin. Cancer Res., 2018-02-14;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. 2DL1, 2DL2 and 2DL3 all contribute to KIR phenotype variability on human NK cells.
    Authors: Dunphy S, Guinan K, Chorcora C, Jayaraman J, Traherne J, Trowsdale J, Pende D, Middleton D, Gardiner C
    Genes Immun, 2015-05-07;16(5):301-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells.
    Authors: Marquardt N, Beziat V, Nystrom S, Hengst J, Ivarsson M, Kekalainen E, Johansson H, Mjosberg J, Westgren M, Lankisch T, Wedemeyer H, Ellis E, Ljunggren H, Michaelsson J, Bjorkstrom N
    J Immunol, 2015-02-11;194(6):2467-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Licensed human natural killer cells aid dendritic cell maturation via TNFSF14/LIGHT.
    Authors: Holmes T, Wilson E, Black E, Benest A, Vaz C, Tan B, Tanavde V, Cook G
    Proc Natl Acad Sci U S A, 2014-12-15;111(52):E5688-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Modulation of the natural killer cell KIR repertoire by cytomegalovirus infection.
    Authors: Charoudeh H, Terszowski G, Czaja K, Gonzalez A, Schmitter K, Stern M
    Eur J Immunol, 2012-12-12;43(2):480-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Molecular characterisation of KIR2DS2*005, a fusion gene associated with a shortened KIR haplotype.
    Authors: Ordonez D, Gomez-Lozano N, Rosales L, Vilches C
    Genes Immun., 2011-05-19;12(7):544-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus.
    Authors: Bjorkstrom NK, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, Michaelsson J, Malmberg KJ, Klingstrom J, Ahlm C, Ljunggren HG
    J. Exp. Med., 2010-12-20;208(1):13-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. NK cell terminal differentiation: correlated stepwise decrease of NKG2A and acquisition of KIRs.
    Authors: Beziat V, Descours B, Parizot C, Debre P, Vieillard V
    PLoS ONE, 2010-08-06;5(8):e11966.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human KIR2DL3/CD158b2 Fluorescein-conjugated Antibody
By Perri Callaway on 07/17/2017
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human
Flow Cytometry Human KIR2DL3/CD158b2 Fluorescein-conjugated Antibody FAB2014F