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Human KIR2DS4/CD158i APC-conjugated Antibody

Catalog #: FAB1847A
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1847A-025
FAB1847A-100
Detection of KIR2DS4/CD158i in Human PBMCs by Flow Cytometry.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human KIR2DS4/CD158i APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects KIR2DS4/CD158i in Western blots. In flow cytometry, this antibody stains cells transfected with KIR2DS4/CD158i. Does not stain cells transfected with KIR2DL1, 2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 3DL1, 3DL2, or 3DS1.
Source
Monoclonal Mouse IgG2A Clone # 179315
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human KIR2DS4
Accession # P43632
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of KIR2DS4/CD158i antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of KIR2DS4/CD158i in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human KIR2DS4/CD158i APC‑conjugated Monoclonal Antibody (Catalog # FAB1847A) and Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KIR2DS4/CD158i

KIR2DS4 is an activating immunoglobulin-like receptor expressed on NK cells and some T cells. It has been implicated in the recognition of some HLA-C alleles (1). A deletion variant encoding a single domain secreted isoform of KIR2DS4 has also been described (2).

References
  1. Katz, G. et al. (2001) J. Immunol. 166:7260.
  2. Maxwell, L. et al. (2002) Tissue Antigens 60:254.
Long Name
Killer Cell Immunoglobulin-like Receptor, Two Domains, Short Cytoplasmic Tail, 4
Entrez Gene IDs
3809 (Human)
Alternate Names
CD158 antigen-like family member I; CD158i antigen; CD158i; cl-39; killer cell immunoglobulin-like receptor 2DS4; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail4,nkat8; killer inhibitory receptor 4-1-2; KIR antigen 2DS4; KIR2DS4; KKA3KIR1D; MGC120019; MGC125315; MGC125317; MHC class I NK cell receptor; natural killer cell inhibitory receptor; Natural killer-associated transcript 8; NKAT-8; NKAT8KIR412; P58 natural killer cell receptor clones CL-39/CL-17; p58 NK receptor CL-39/CL-17

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Citations for Human KIR2DS4/CD158i APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Complex Autoinflammatory Syndrome Unveils Fundamental Principles of JAK1 Kinase Transcriptional and Biochemical Function
    Authors: Conor N. Gruber, Jorg J.A. Calis, Sofija Buta, Gilad Evrony, Jerome C. Martin, Skyler A. Uhl et al.
    Immunity
  2. Decitabine Inhibits Gamma Delta T Cell Cytotoxicity by Promoting KIR2DL2/3 Expression
    Authors: Chao Niu, Min Li, Shan Zhu, Yongchong Chen, Lei Zhou, Dongsheng Xu et al.
    Frontiers in Immunology
  3. 2DL1, 2DL2 and 2DL3 all contribute to KIR phenotype variability on human NK cells.
    Authors: Dunphy S, Guinan K, Chorcora C, Jayaraman J, Traherne J, Trowsdale J, Pende D, Middleton D, Gardiner C
    Genes Immun, 2015-05-07;16(5):301-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. CMV drives clonal expansion of NKG2C+ NK cells expressing self-specific KIRs in chronic hepatitis patients.
    Authors: Beziat V, Dalgard O, Asselah T, Halfon P, Bedossa P, Boudifa A, Hervier B, Theodorou I, Martinot M, Debre P, Bjorkstrom NK, Malmberg KJ, Marcellin P, Vieillard V
    Eur. J. Immunol., 2011-12-16;42(2):447-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. NK cell terminal differentiation: correlated stepwise decrease of NKG2A and acquisition of KIRs.
    Authors: Beziat V, Descours B, Parizot C, Debre P, Vieillard V
    PLoS ONE, 2010-08-06;5(8):e11966.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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