Human Klotho beta Antibody Summary
Phe53-Leu997
Accession # Q86Z14
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Klotho beta by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Klotho beta Monoclonal Antibody (Catalog # MAB5889) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Klotho beta at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Klotho beta by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Klotho beta at approximately 177/170 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Klotho beta Monoclonal Antibody (Catalog # MAB5889). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Klotho beta
Klotho beta (b-Klotho or KLB) is a 125-135 kDa member of the KL family, Glycosyl Hydrolase 1 superfamily of proteins. It plays key role in the biology of the endocrine FGFs (FGF-15/19; 21; 23). KLB appears to serve as a signaling cofactor for select FGFRs, including FGFR-1c, -2c, -3c and FGFR4. The cofactor is known to constitutively interact with FGFRs, promote FGF binding to their appropriate FGFRs, and to bind directly to FGF-19 and FGF-21. Notably, FGF-23 appears to utilize a-Klotho/KL for its FGFR signaling cofactor, and FGF-1, while not dependent upon KLB for signaling, can utilize KBL to potentiate certain activities. KLB is expressed on select cells, including adipocytes and hepatocytes. Human KLB is a 1044 amino acids (aa) type III transmembrane (TM) protein (i.e.-a type I TM protein lacking a signal sequence). It contains a 996 aa extracellular region (aa 1-996) and a 27 aa cytoplasmic domain (aa 1018-1044). The extracellular region contains two glycosyl hydrolase domains (aa 77-508 and 517-967) plus eleven potential N-linked glycosylation sites. Neither hydrolase domain is active as they lack a key Glu residue needed for activity. Over aa 53-997, human KLB shares 80% aa sequence identity with mouse KLB. And relative to KL/a-Klotho, human KLB and KL share just 44% aa sequence identity over the entire length of the molecules.
Product Datasheets
Citations for Human Klotho beta Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Subcutaneous delivery of FGF21 mRNA therapy reverses obesity, insulin resistance, and hepatic steatosis in diet-induced obese mice
Authors: Stefano Bartesaghi, Kristina Wallenius, Daniel Hovdal, Mathias Liljeblad, Simonetta Wallin, Niek Dekker et al.
Molecular Therapy - Nucleic Acids
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FGFR redundancy limits the efficacy of FGFR4-selective inhibitors in hepatocellular carcinoma
Authors: Z Tao, Y Cui, X Xu, T Han
Proceedings of the National Academy of Sciences of the United States of America, 2022-09-30;119(40):e2208844119.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A systematic dissection of sequence elements determining ?-Klotho and FGF interaction and signaling
Authors: SY Shi, YW Lu, J Richardson, X Min, J Weiszmann, WG Richards, Z Wang, Z Zhang, J Zhang, Y Li
Sci Rep, 2018-07-23;8(1):11045.
Applications: ELISA Development (Detection)
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