Human Kremen-2 Antibody

Catalog #: MAB1946 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1946
MAB1946-SP

All Kremen-2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Kremen-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Kremen-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Kremen-1 or recombinant mouse Kremen-2 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 276709

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kremen-2
Gln19-Ala364
Accession # Q8NCW0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Kremen‑2 (Catalog # 1946-KR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kremen-2

Kremen (Kringle-containing protein marking the eye and the nose) proteins are type I transmembrane proteins that contain extracellular kringle, WSC and CUB domains and an intracellular region without any conserved motifs (1). Two related members, Kremen-1 and -2, have been identified. Kremens bind a subset of the secreted Dickkopf (Dkk) proteins (Dkk-1, -2, and -4) with high affinity to modulate the canonical Wnt signaling pathway that is transduced by the ternary receptor complex composed of Wnt, the seven-transmembrane domain receptor Frizzled, and the LDL-receptor-related protein 5/6 (LRP5/6) coreceptor (2, 3). Within the Dkk family, Dkk-1 and -4 bind directly to the LRP5/6 coreceptor to antagonize the canonical Wnt/ beta -catenin signaling pathway, but not the planar cell polarity (PCP) signaling pathway that does not involve LRP5/6 (4). In contrast, Dkk-3 has no effect on Wnt signaling and Dkk-2 can function either as an LRP agonist or antagonist, depending on whether the cell expresses Kremen (5). Kremen cooperates with Dkk to antagonize Wnt signaling via formation of a Kremen-Dkk-LRP ternary complex that triggers the internalization and clearance of the complex from the cell surface (3). All three extracellular domains but not the cytoplasmic region of a membrane anchored Kremen are needed for binding to the second cysteine-rich domain of Dkks (3). Human Kremen-2 cDNA encodes a 462 amino acid (aa) glycosylated protein with a putative 18 aa signal peptide, a 353 aa extracellular domain, a 17 aa transmembrane domain and a 75 aa cytoplasmic domain. In the extracellular domain, it shares 88% and 42% amino acid sequence identity with mouse Kremen-2 and human Kremen-1, respectively.

References

Nakamura, T. et al. (2001) Biochim. Biophys. Acta 1518:63.
Davidson G. et al. (2002) Development 129:5587.
Mao, B. et al. (2002) Nature 417:664.
Zorn, A.M. (2001) Curr. Biol. 11:R592.
Mao, B. and C. Niehrs (2003) Gene 302:179.

Long Name

Kringle-containing Transmembrane Protein 2

Entrez Gene IDs

79412 (Human); 73016 (Mouse)

Alternate Names

Dickkopf receptor 2; kremen protein 2; Kremen2; Kremen-2; kringle containing transmembrane protein 2; Kringle domain-containing transmembrane protein 2; KRM2Kringle-containing protein marking the eye and the nose; MGC10791; MGC16709