Human LAIR2 Antibody

Catalog #: AF2665 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2665
AF2665-SP

All LAIR2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LAIR2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LAIR2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human LAIR1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LAIR2
Gln22-Pro152
Accession # Q6ISS4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human LAIR2 (Catalog # 2665-LR)

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAIR2

LAIR-2 (leukocyte-associated Ig-like receptor-2; CD306) is a secreted, 131 amino acid (aa) protein that contains one Ig-like C2 type domain, making it a member of the Ig superfamily. When compared to LAIR-1, its transmembrane counterpart, it shares 83% aa identity across the signal sequence and extracellular domains; (1‑3) although one is secreted and one is membrane-bound, the two LAIR proteins are thought to have arisen from a common gene ancestor and appear to share similar adhesion profiles. This suggests that LAIR-2 may compete with LAIR-1 for ligand binding (3, 4). A 114 aa alternate splice form of LAIR-2 is truncated at the C‑terminus, but retains the entire Ig domain (1‑3). The expression profile of these splice forms, and the presence of orthologs in other species, have not been reported.

References

Meyaard, L. (2003) J. Biol. Regul. Homeost. Agents 17:330.
Meyaard, L. et al. (1999) J. Immunol. 162:5800.
Meyaard, L. et al. (1997) Immunity 7:283.
Xu, X.G. et al. (2005) Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 21:553.

Long Name

Leukocyte-associated Ig-like Receptor 2

Entrez Gene IDs

3904 (Human); 102141809 (Cynomolgus Monkey)

Alternate Names

CD306 antigen; CD306; CD306leukocyte-associated Ig-like receptor-2; LAIR2; LAIR-2; leukocyte-associated Ig-like receptor 2; leukocyte-associated immunoglobulin-like receptor 2; MGC71634