Human LAMP-1/CD107a APC-conjugated Antibody

Catalog #: IC4800A Datasheet / COA / SDS

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IC4800A

Detection of LAMP‑1/CD107a in Human NK cells by Flow Cytometry.

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Human LAMP-1/CD107a APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human LAMP-1/CD107a in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 508921

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LAMP-1/CD107a
Ala28-Asn380
Accession # P11279

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of LAMP‑1/CD107a in Human NK cells by Flow Cytometry. Human NK Cells either (A) stimulated in a 1:1 ratio with K562 human chronic myelogenous leukemia cell line and 3 µM monensin for 5 hours or (B) unstimulated were stained with Mouse Anti-Human LAMP-1/CD107a APC-conjugated Monoclonal Antibody (Catalog # IC4800A) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Intracellular Staining by Flow Cytometry

Detection of LAMP-1/CD107a antibody in THP-1 Human Cell Line antibody by Flow Cytometry.

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Detection of LAMP-1/CD107a in THP‑1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human LAMP-1/CD107a APC-conjugated Monoclonal Antibody (Catalog # IC4800A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: LAMP-1/CD107a

Lysosome-Associated Membrane Protein-1 (LAMP-1), also known as CD107a, is a 100-130 kDa member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T-cells, and with LAMP-2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP-1 is a 389 amino acid (aa) type I transmembrane glycoprotein. It contains a 354 aa luminal/extracellular domain (ECD) (aa 28-381) and a 12 aa cytoplasmic tail (aa 405-416). The ECD has two large looping regions (aa 28-193 and 227-381) plus multiple N- and O-linked glycosylation sites. There is one potential splice variant that shows a 26 aa substitution in the signal sequence. Over aa 28-380, human LAMP-1 shares 64% aa identity with mouse LAMP-1.

Long Name

Lysosome-associated Membrane Glycoprotein 1

Entrez Gene IDs

3916 (Human); 16783 (Mouse)

Alternate Names

CD107 antigen-like family member A; CD107a antigen; CD107a; LAMP1; LAMP-1; LAMPA; LGP120; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1