Human LAMP1/CD107a Biotinylated Antibody

Catalog #: BAM4800 Datasheet / COA / SDS

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BAM4800

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Human LAMP1/CD107a Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

LAMP1 antibodies are ideal for immunocytochemistry colocalization studies in lysosomes. Detects human LAMP1/CD107a in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 508921

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LAMP1/CD107a
Ala28-Asn380
Accession # P11279

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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LAMP1/CD107a in HeLa Human Cell Line. LAMP1/CD107a was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human LAMP1/CD107a Biotinylated Monoclonal Antibody (Catalog # BAM4800) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (orange; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAMP-1/CD107a

Lysosome-associated membrane protein-1 (LAMP1), also known as CD107a, is a 100‑130 kDa member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T-cells, and with LAMP2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP1 is a 389 amino acid (aa) type I transmembrane glycoprotein. It contains a 354 aa luminal/extracellular domain (ECD) (aa 28‑381) and a 12 aa cytoplasmic tail (aa 405‑416). The ECD has two large looping regions (aa 28‑193 and 227‑381) plus multiple N- and O-linked glycosylation sites. There is one potential splice variant that shows a 26 aa substitution in the signal sequence. Over aa 28‑380, human LAMP1 shares 64% aa identity with mouse LAMP1.

Long Name

Lysosome-associated Membrane Glycoprotein 1

Entrez Gene IDs

3916 (Human); 16783 (Mouse)

Alternate Names

CD107 antigen-like family member A; CD107a antigen; CD107a; LAMP1; LAMP-1; LAMPA; LGP120; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1