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Human Langerin/CD207 Antibody

Catalog #: AF2088
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2088
AF2088-SP
Langerin/CD207 in Human Skin.
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Citations (11)
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Human Langerin/CD207 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Langerin/CD207 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Langerin/CD207
Tyr64-Pro328
Accession # Q9UJ71
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Langerin/CD207 (Catalog # 2088-LN)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Langerin/CD207 antibody in Human Skin by Immunohistochemistry (IHC-P). View Larger

Langerin/CD207 in Human Skin. Langerin/CD207 was detected in immersion fixed paraffin-embedded sections of human skin using 15 µg/mL Goat Anti-Human Langerin/CD207 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2088) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of Langerhans cells in the skin epidermis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Langerin/CD207

Langerin (also known as CD207) is a type II transmembrane glycoprotein which is member K of the C-type lectin domain family 4 (1). Langerin is used as a marker for Langerhans cells (LCs) which represent the immature dendritic cells in the epidermis (1, 2). LCs uniquely contain “tennis racket”-shaped endosomal recycling compartment subdomains with pentalamellar membranes termed Birbeck granules (1-3). Langerin is necessary and sufficient for Birbeck granule formation (1). The 328 amino acid (aa) human langerin sequence contains a 43 aa cytoplasmic domain, a 21 aa transmembrane domain and a 264 aa extracellular domain (ECD) that contains a coiled-coil domain and a single C-type lectin domain. Trimerization greatly increases the lectin binding affinity (4). Langerin internalizes endogenous proteins such as type I procollagen. Internalization by LC is thought to lead to suppression of self reactions (4-6). Langerin also mediates endocytosis of non-peptide antigens containing mannose, N-acetyl glucosamine and fucose that are expressed by mycobacteria and fungae (4, 7). Some antigens, such as the M. leprae glycolipid arabinomycolate, are ultimately presented by human LC CD1a in cutaneous-draining lymph nodes (8). Langerin performs a barrier-like function to HIV-1 transmission due to its internalization of virus particles for destruction (9). A rare human polymorphism within the lectin domain, W264R, abolishes both carbohydrate recognition and Birbeck granule formation (10, 11). Genetic deletion of mouse langerin was not shown to have functional consequence other than abolishing Birbeck granule formation (12). Human langerin shares 68%, 62%, 71% aa identity with mouse, rat, and bovine langerin ECD, respectively.

References
  1. Valladeau, J. et al. (2000) Immunity 12:71. 
  2. Valladeau, J. et al. (2003) Immunol. Res. 28:93. 
  3. McDermott, R. et al. (2002) Mol. Biol. Cell 13:317. 
  4. Stambach, N.S. and M.E. Taylor (2003) Glycobiology 13:401. 
  5. Tada, Y. et al. (2006) J. Invest. Dermatol. 126:1549. 
  6. Ritter, U. and A. Osterloh (2007) Med. Microbiol. Immunol. 196:51. 
  7. Takahara, K. et al. (2003) Int. Immunol. 16:819.
  8. Hunger, R.E. et al. (2004) J. Clin. Invest. 113:701.
  9. De Witte, L. et al. (2007) Nat. Med. 13:367.
  10. Verdijk, P. et al. (2005) J. Invest. Dermatol. 124:714.
  11. Ward, E.M. et al. (2006) J. Biol. Chem. 281:15450.
  12. Kissenpfennig, A. et al. (2005) Mol. Cell. Biol. 25:88.
Entrez Gene IDs
50489 (Human); 246278 (Mouse)
Alternate Names
CD207 antigen; CD207 antigen, langerin; CD207 molecule, langerin; CD207; CLEC4KLangerhans cell specific c-type lectin; C-type lectin domain family 4 member K; C-type lectin domain family 4, member K; Langerin

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Citations for Human Langerin/CD207 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Detection of Intraepithelial and Stromal Langerin and CCR5 Positive Cells in the Human Endometrium: Potential Targets for HIV Infection
    Authors: Tove Kaldensjö, Pernilla Petersson, Anna Tolf, Gareth Morgan, Kristina Broliden, Taha Hirbod
    PLoS ONE
  2. CGRP inhibits human Langerhans cells infection with HSV by differentially modulating specific HSV-1 and HSV-2 entry mechanisms
    Authors: E Cohen, J Mariotton, F Rozenberg, A Sams, TH van Kuppev, N Barry Delo, M Zerbib, M Bomsel, Y Ganor
    Mucosal Immunology, 2022-05-13;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Herpes Simplex Virus type 1 infects Langerhans cells and the novel epidermal dendritic cell, Epi-cDC2s, via different entry pathways
    Authors: KM Bertram, NR Truong, JB Smith, M Kim, KJ Sandgren, KL Feng, JJ Herbert, H Rana, K Danastas, M Miranda-Sa, JW Rhodes, E Patrick, RC Cohen, J Lim, SL Merten, AN Harman, AL Cunningham
    PloS Pathogens, 2021-04-27;17(4):e1009536.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. The Essential Role of anxA2 in Langerhans Cell Birbeck Granules Formation
    Authors: SM Thornton, VD Samararatn, JG Skeate, C Buser, KP Lühen, JR Taylor, DM Da Silva, WM Kast
    Cells, 2020-04-15;9(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunoelectron Microscopy
  5. Identification of HIV transmitting CD11c+ human epidermal dendritic cells
    Authors: KM Bertram, RA Botting, H Baharlou, JW Rhodes, H Rana, JD Graham, E Patrick, J Fletcher, TM Plasto, NR Truong, C Royle, CM Doyle, O Tong, N Nasr, L Barnouti, MP Kohout, AJ Brooks, MP Wines, P Haertsch, J Lim, MP Gosselink, G Ctercteko, JD Estes, MJ Churchill, PU Cameron, E Hunter, MA Haniffa, AL Cunningham, AN Harman
    Nat Commun, 2019-06-21;10(1):2759.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. TRIM5? Restricts Flavivirus Replication by Targeting the Viral Protease for Proteasomal Degradation
    Authors: AI Chiramel, NR Meyerson, KL McNally, RM Broeckel, VR Montoya, O Méndez-Sol, SJ Robertson, GL Sturdevant, KJ Lubick, V Nair, BH Youseff, RM Ireland, CM Bosio, K Kim, J Luban, VM Hirsch, RT Taylor, F Bouamr, SL Sawyer, SM Best
    Cell Rep, 2019-06-11;27(11):3269-3283.e6.
  7. Chemokine Levels in the Penile Coronal Sulcus Correlate with HIV-1 Acquisition and Are Reduced by Male Circumcision in Rakai, Uganda
    PLoS Pathog, 2016-11-29;12(11):e1006025.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. In situ distribution of HIV-binding CCR5 and C-type lectin receptors in the human endocervical mucosa.
    Authors: Hirbod T, Kaldensjo T, Broliden K
    PLoS ONE, 2011-09-30;6(9):e25551.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  9. Plasmacytoid dendritic cells infiltrate the skin in positive tuberculin skin test indurations.
    Authors: Bond E, Liang F, Sandgren KJ, Smed-Sorensen A, Bergman P, Brighenti S, Adams WC, Betemariam SA, Rangaka MX, Lange C, Wilkinson RJ, Andersson J, Lore K
    J. Invest. Dermatol., 2011-08-18;132(1):114-23.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  10. HIV-1 replication in Langerhans and interstitial dendritic cells is inhibited by neutralizing and Fc-mediated inhibitory antibodies.
    Authors: Peressin M, Holl V, Schmidt S, Decoville T, Mirisky D, Lederle A, Delaporte M, Xu K, Aubertin AM, Moog C
    J. Virol., 2010-11-17;85(2):1077-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Stable CD4 Expression and Local Immune Activation in the Ectocervical Mucosa of HIV-Infected Women
    Authors: Taha Hirbod, Joshua Kimani, Annelie Tjernlund, Juliana Cheruiyot, Anna Petrova, Terry B. Ball et al.
    The Journal of Immunology

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