Human LAR Antibody

Catalog #: MAB3004 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3004
MAB3004-SP

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Human LAR Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human LAR in Western blots. In Western blots, this antibody detects both full-length protein and the cleaved LAR extracellular domain (ECD). It does not cross-react with recombinant human PTPRM, PTPRK, DEP1, or PTPR gamma.

Source

Monoclonal Rat IgG₁ Clone # 384727

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LAR
Ala27-Glu1251
Accession # NP_569707

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human LAR by Western Blot. Western blot shows lysates of CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human LAR Monoclonal Antibody (Catalog # MAB3004) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for LAR at approximately 139 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAR

Leukocyte Antigen-Related (LAR) tyrosine phosphatase, also known as Protein Tyrosine Phosphatase, Receptor-type F (PTPRF), is an integral membrane protein with anon-glycosylated molecular weight of 207 kDa. The extracellular domain is cleaved near the cell membrane by a subtilisin-like endoprotease to a molecular weight of 139 kDa. Depending on cellular conditions, the extracellular domain may remain associated with the rest of the molecule or can be shed into the extracellular medium.

Long Name

Leukocyte Antigen-Related Tyrosine Phosphatase

Entrez Gene IDs

5792 (Human); 104121 (Mouse)

Alternate Names

LAR; LCA-homolog; protein tyrosine phosphatase, receptor type, F; PTPRF; receptor type, F polypeptide; receptor-linked protein-tyrosine phosphatase LAR; receptor-type tyrosine-protein phosphatase F