Human Lck Antibody

Detection of Human Lck by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Lck Monoclonal Antibody (Catalog # MAB37041) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Lck at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Lck in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human Lck Monoclonal Antibody (Catalog # MAB37041) followed by Phycoery-thrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A). Quadrant markers were set based on control antibody staining (MAB003).

Lck in Human PBMCs. Lck was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human Lck Monoclonal Antibody (Catalog # MAB37041) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Human Lck by Simple Western^TM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Lck at approximately 57 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human Lck Monoclonal Antibody (Catalog # MAB37041). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.