Human LEF1 Antibody

Catalog #: AF7647
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7647
AF7647-SP

Detection of Human LEF1 by Western Blot.

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All LEF1 Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LEF1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LEF1 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human (rh) TCF-1, rhTCF-2, and rhTCF-4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human LEF1
Met120-Met280 (predicted)
Accession # Q9UJU2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Immunocytochemistry

1-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human LEF1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human LEF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7647) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for LEF1 at approximately 35-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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LEF1 in Neuro‑2A Mouse Cell Line. LEF1 was detected in immersion fixed Neuro-2A mouse neuroblastoma cell line using Goat Anti-Human LEF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7647) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LEF1

LEF1 (Lymphoid Enhancer-binding Factor 1) is a functionally diverse member of the TCF/LEF family of transcription factors. The name LEF1 was originally applied to the mouse factor, while its human counterpart was named TCF-1 alpha. Since there is a related molecule named TCF-1, to avoid confusion with TCF-1 alpha, both species factors are now called LEF1. Although its predicted MW is 44 kDa, it runs anomalously at 35-55 kDa in SDS-PAGE. LEF1 has restricted expression in adult, being limited to T cells, thymocytes, pre-B cells and NK cells. LEF1 demonstrates sequence-specific (CCTTTG[T/A][T/A]) DNA binding and directs beta -catenin to Wnt‑responsive genes. Either transcriptional activation or repression may occur on LEF1 target genes, depending upon 1) the cofactors recruited to the LEF1: beta ‑catenin complex, and 2) the phosphorylation state of LEF1. Human LEF1 is 399 amino acids (aa) in length. It contains one DNA-binding HMG-box (aa 298‑369), three potential phosphorylation sites, and an N-terminal SUMOylation site at Lys27. There are at least five isoform variants. One utilizes an alternative start site at Met116, a second contains a 47 aa substitution for aa 283‑299, and a third combines the previous two variations. A fourth variant shows a deletion of aa 214‑241, while a fifth variant combines the afore mentioned deletion of aa 214-241 with a 25 aa substitution for aa 390‑399. Over aa 120‑280, human LEF1 shares 97% aa sequence identity with mouse LEF1.

Long Name

Lymphoid Enhancer-binding Factor 1

Entrez Gene IDs

51176 (Human); 16842 (Mouse); 161452 (Rat)

Alternate Names

DKFZp586H0919; FLJ46390; LEF1; LEF-1; lymphoid enhancer-binding factor 1; T cell-specific transcription factor 1-alpha; TCF10; TCF1ALPHA; TCF1-alpha; TCF7L3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human LEF1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Gut Microbiota Modulate CD8ï¿½T Cell Responses to Influence Colitis-Associated Tumorigenesis
Authors: AI Yu, L Zhao, KA Eaton, S Ho, J Chen, S Poe, J Becker, A Gonzalez, D McKinstry, M Hasso, J Mendoza-Ca, J Whitfield, C Koumpouras, PD Schloss, EC Martens, GY Chen
Cell Rep, 2020-04-07;31(1):107471.
Species: Human
Sample Types: Whole Cells
Applications: ICC
The osteogenic differentiation of human adipose-derived stem cells is regulated through the let-7i-3p/LEF1/beta-catenin axis under cyclic strain
Authors: Y Luo, R Ge, H Wu, X Ding, H Song, H Ji, M Li, Y Ma, S Li, C Wang, H Du
Stem Cell Res Ther, 2019-11-21;10(1):339.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot