Home / Legumain/Asparaginyl Endopeptidase / Human Legumain/Asparaginyl Endopeptidase Antibody

Human Legumain/Asparaginyl Endopeptidase Antibody

Catalog #: MAB2199
2 Citations Datasheet / COA / SDS
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MAB2199
MAB2199-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Legumain/Asparaginyl Endopeptidase Antibody Summary

Species Reactivity
Human
Specificity
Detects human Legumain/Asparaginyl Endopeptidase (aa18‑433) in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Legumain is observed. Recognizes the pro form of human Legumain and does not recognize the mature form (aa 18‑323).
Source
Monoclonal Mouse IgG2A Clone # 312114
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0-derived recombinant human Legumain/Asparaginyl Endopeptidase
Ile18-Tyr433
Accession # Q99538
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Legumain (Catalog # 2199‑CY) under non-reducing conditions only
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Legumain (Catalog # 2199‑CY), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Legumain/Asparaginyl Endopeptidase

Legumain is a lysosomal cysteine protease whose activity is found in several tissues tested (1, 2). Legumain plays a pivotal role in the endosomal/lysosomal degradation system because the Legumain deficiency causes the accumulation of pro cathepsins B, H and L, another group of lysosomal cysteine proteases (3). Over-expression of Legumain in tumors is significant for invasion/metastasis (4). Also known as Asparaginyl Endopeptidase, it specifically cleaves peptide bonds with Asn at the P1 position. Nevertheless, it also cleaves peptide bonds with Asp at the P1 position. Auto-activation of pro Legumain involves both types of the cleavage, which result in the removal of the pro peptides in both C- and N-termini (5). In addition, Legumain activates pro MMP-2 and processes bacterial antigens for MHC class II presentation and pro thymosin alpha to thymosin alpha 1 and thymosin alpha 11, two acidic peptides with immunoregulatory properties (6‑8). Human Legumain is synthesized as a 433 amino acid precursor with a signal peptide (residues 1‑17) and a pro enzyme (residues 18‑433). The activity of Legumain can be inhibited by Cystatins C and E/M.

References
  1. Chen, J.-M. et al. (1997) J. Biol. Chem. 272:8090.
  2. Tanaka, T. et al. (1996) Cytogenet. Cell Genet. 74:120.
  3. Shirahama-Noda, K. et al. (2003) J. Biol. Chem. 278:33194.
  4. Liu, C. et al. (2003) Cancer Res. 63: 2957.
  5. Li D.N. et al. (2003) J. Biol. Chem. 278:38980.
  6. Chen, J.M. et al. (2001) Biol. Chem. 382:777.
  7. Schwarz, G. et al. (2002) Biol. Chem. 383:1813.
  8. Sarndeses, C.S. et al. (2003) J. Biol. Chem. 278:13286.
Entrez Gene IDs
5641 (Human); 19141 (Mouse)
Alternate Names
AEP; Asparaginyl Endopeptidase; cysteine protease 1; Legumain; LGMN; LGMN1; Protease, cysteine 1; protease, cysteine, 1 (legumain); PRSC1EC 3.4.22.34

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Citations for Human Legumain/Asparaginyl Endopeptidase Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Novel candidate genes in unstable areas of human atherosclerotic plaques.
    Authors: Papaspyridonos M, Smith A, Burnand KG, Taylor P, Padayachee S, Suckling KE, James CH, Greaves DR, Patel L
    Arterioscler. Thromb. Vasc. Biol., 2006-06-01;26(8):1837-44.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Asparaginyl endopeptidase improves the resistance of microtubule-targeting drugs in gastric cancer through IQGAP1 modulating the EGFR/JNK/ERK signaling pathway
    Authors: Y Cui, Q Li, H Li, Y Wang, H Wang, W Chen, S Zhang, J Cao, T Liu
    Onco Targets Ther, 2017-02-03;10(0):627-643.

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