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Human LEKTI/SPINK5 Antibody

Catalog #: AF8515
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF8515
AF8515-SP
Detection of Human SPINK5 by Western Blot.
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Citations (1)
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Human LEKTI/SPINK5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SPINK5 in direct ELISAs and Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Human embyronic kidney cell line HEK293-derived recombinant human SPINK5
Glu626-Glu1064
Accession # Q9NQ38
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human SPINK5 antibody by Western Blot. View Larger

Detection of Human SPINK5 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human SPINK5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8515) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SPINK5 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LEKTI/SPINK5

LEKTI (Lympho-epithelial Kazal-type-related inhibitor; also SPINK5, Serine Protease Inhibitor Kazal-type 5) is a 150 kDa precursor protein that is cleaved into the following 2 chains: Hemofiltrate peptide HF6478 and Hemofiltrate peptide HF7665. LEKTI is a multidomain serine protease inhibitor that contains 15 Kazal-like inhibitory domains. It may play a role in skin and hair morphogenesis and anti-inflammatory and/or antimicrobial protection of mucous epithelia. Mutations may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. It contains fifteen Kazal-like domains. Mature human SPINK5 shares 60% aa sequence identity with mouse SPINK5.

Long Name
Serine Peptidase Inhibitor, Kazal Type 5
Entrez Gene IDs
11005 (Human); 72432 (Mouse); 361319 (Rat)
Alternate Names
Kazal type 5; LEKTI; LETKI; NETS; serine peptidase inhibitor, Kazal type 5; SPINK5; VAKTI

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Citation for Human LEKTI/SPINK5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. LncRNA miR205HG hinders HNRNPA0 translation: anti‐oncogenic effects in esophageal carcinoma
    Authors: Xiaoying Dong, Xuyuan Chen, Di Lu, Dingwei Diao, Xiguang Liu, Shijie Mai et al.
    Molecular Oncology

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