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Human Leukotriene B4 R1 Antibody

Catalog #: MAB099
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB099
MAB099-SP
Detection of Leukotriene B4 R1 in Human Blood Monocytes by Flow Cytometry.
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Citations (3)
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Human Leukotriene B4 R1 Antibody Summary

Species Reactivity
Human
Specificity
Recognizes human Leukotriene B4 R1 (Pettersson, A. et al. (2000) Biochem. Biophys. Res. Commun. 279:520).
Source
Monoclonal Mouse IgG1 Clone # 203/14F11
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HeLa cervical epithelial carcinoma cell line transfected with human Leukotriene B4 R1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Leukotriene B4 R1 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of Leukotriene B4 R1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monoctyes were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human Leukotriene B4 R1 Monoclonal Antibody (Catalog # MAB099) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Leukotriene B4 R1

Polymorphonuclear granulocytes secrete the lipid chemotactic mediator Leukotriene B4 (LTB4) in response to inflammatory stimuli (1). Neutrophils, monocytes and lymphocytes respond to LTB4 via specific receptors localized on the cell surface (2-4). The high affinity LTB4 Receptor known as BLT1 is only expressed on leukocytes (5-7) while a second low affinity receptor BLT2 is expressed more ubiquitously (8, 9). The BLT1 and BLT2 are G-protein linked seven-transmembrane spanning receptors that share about 37-45% amino acid identity (8, 9).

References
  1. Samuelsson, B. et al. (1987) Science 237:1171.
  2. Brom, J. and W. Konig (1989) Immunology 68:479.
  3. Patry, C. et al. (1996) Prostaglandins, Leukotrienes and Essential Fatty Acids 54:361.
  4. Dasari, V.R. et al. (2000) Immunopharm. 48:157.
  5. Pettersson, A. et al. (2000) Biochem. Biophys. Res. Comm. 279:520.
  6. Yokomizo, T. et al. (1997) Nature 387:620.
  7. Kato, K. et al. (2000) J. Exp. Med. 192:413.
  8. Yokomizo, T. et al. (2000) J. Exp. Med. 192:421.
  9. Kamohara, M. et al. (2000) J. Biol. Chem. 275:27000.
Long Name
Leukotriene B4 Receptor 1
Entrez Gene IDs
1241 (Human)
Alternate Names
BLT1; chemokine receptor-like 1; CMKRL1; Leukotriene B4 R1; leukotriene B4 receptor; Leukotriene B4R1; LTB4R; LTB4R1; LTBR1; P2Y purinoceptor 7; purinergic receptor P2Y, G-protein coupled, 7

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Citations for Human Leukotriene B4 R1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Development and application of a high-content virion display human GPCR array
    Authors: Guan-Da Syu, Shih-Chin Wang, Guangzhong Ma, Shuang Liu, Donna Pearce, Atish Prakash et al.
    Nature Communications
  2. Low leukotriene B4 receptor 1 leads to ALOX5 downregulation at diagnosis of chronic myeloid leukemia.
    Authors: Lucas C, Harris R, Giannoudis A, McDonald E, Clark R
    Haematologica, 2014-09-05;99(11):1710-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
    Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
    Immunol. Lett., 2010-10-07;134(2):167-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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