Human Leukotriene B4 R1 Fluorescein-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Leukotriene B4 R1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human Leukotriene B4 R1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB099F) or (B) Mouse IgG1Fluorescein Isotype Control (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Leukotriene B4 R1
Polymorphonuclear granulocytes secrete the lipid chemotactic mediator Leukotriene B4 (LTB4) in response to inflammatory stimuli (1). Neutrophils, monocytes and lymphocytes respond to LTB4 via specific receptors localized on the cell surface (2-4). The high affinity LTB4 Receptor known as BLT1 is only expressed on leukocytes (5-7) while a second low affinity receptor BLT2 is expressed more ubiquitously (8, 9). The BLT1 and BLT2 are G-protein linked seven-transmembrane spanning receptors that share about 37-45% amino acid identity (8, 9). Enhanced LTB4 production and engagement of the BLT receptors can be important in allergic and inflammatory diseases such as asthma (10), allergic encephalomyelitis (11), endotoxic shock (12), ischemia (12), psoriasis (13), rheumatoid arthritis (14) and inflammatory bowel disease (15). In addition, it has been reported that BLT1 can function as an additional co-receptor for HIV infection of CD4+ T cells (16, 17). Investigations into the mechanisms and potential inhibitors of LTB4 binding to its’ receptors may provide insight into possible treatment modalities for a number of inflammatory disorders.
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Product Datasheets
Citation for Human Leukotriene B4 R1 Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Selective localization of recognition complexes for leukotriene B4 and formyl-Met-Leu-Phe within lipid raft microdomains of human polymorphonuclear neutrophils.
Authors: Sitrin RG, Emery SL, Sassanella TM, Blackwood RA, Petty HR
J. Immunol., 2006-12-01;177(11):8177-84.
Species: Human
Sample Types: Whole Cells
Applications: FRET
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This antibody was used to stain human peripheral whole blood for analysis by flow cytometry. Neutrophils were identified from total live CD45+ leukocytes and expression of the LTB4 receptor was measured in the FITC channel. Consistent fluorescence is observed after fixation in 4% PFA. Good antibody for staining granulocytes
