Human Lgr6 Antibody

Name: Human Lgr6 Antibody
Brand: R&D Systems
SKU: MAB8458
Rating: 4 (1 reviews)

Catalog #: MAB8458
1 Citation 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8458
MAB8458-SP

Detection of Lgr6 in HEK293 Human Cell Line Transfected with Human Lgr6 and eGFP by Flow Cytometry.

2 Images

All Lgr6 Antibodies

Product Details

Citations (1)

FAQs

Supplemental Products

Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Lgr6 Antibody Summary

Species Reactivity

Human

Specificity

Stains human Lgr6 transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG₁ Clone # 918719

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Lgr6
Met1-Val967
Accession # Q9HBX8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of Lgr6 in HEK293 Human Cell Line Transfected with Human Lgr6 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Lgr6 and eGFP was stained with either (A) Mouse Anti-Human Lgr6 Monoclonal Antibody (Catalog # MAB8458) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Immunocytochemistry

View Larger

Lgr6 in HEK293 Human Cell Line. Lgr6 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with human Lgr6 and eGFP (green) using Mouse Anti-Human Lgr6 Monoclonal Antibody (Catalog # MAB8458) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lgr6

Lgr6 is a glycoprotein hormone receptor phosphorylated upon DNA damage, probably by ATM or ATR. It is a member of the leucine-rich repeat-containing subgroup of the G protein-coupled 7-transmembrane protein superfamily. Lgr6 is a high affinity receptor for R-Spondins 1-3 and potentially functions as a tumor suppressor despite its positive effect on Wnt/ beta -Catenin signaling.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 6

Entrez Gene IDs

59352 (Human); 329252 (Mouse); 498233 (Rat)

Alternate Names

FLJ14471; gonadotropin receptor; GPCR; leucine-rich repeat containing G protein-coupled receptor 6; leucine-rich repeat-containing G protein-coupled receptor 6; leucine-rich repeat-containing G-protein coupled receptor 6; Lgr6; VTS20631

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Peptide Hormone G Protein-Coupled Receptors

Citation for Human Lgr6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1