Human Lgr6 APC-conjugated Antibody

Name: Human Lgr6 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB8458A

Catalog #: FAB8458A Datasheet / COA / SDS

Discontinued Product

FAB8458A has been discontinued.

View all Lgr6 products.

Detection of Lgr6 in HEK293 Human Cell Line Transfected with Human Lgr6 and eGFP by Flow Cytometry.

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Human Lgr6 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Stains human Lgr6 transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG₁ Clone # 918719

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Lgr6
Met1-Val967
Accession # Q9HBX8

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Lgr6 in HEK293 Human Cell Line Transfected with Human Lgr6 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Lgr6 and eGFP was stained with either (A) Mouse Anti-Human Lgr6 APC-conjugated Monoclonal Antibody (Catalog # FAB8458A) or (B) Mouse IgG₁Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Lgr6

Lgr6 is a glycoprotein hormone receptor phosphorylated upon DNA damage, probably by ATM or ATR. It is a member of the leucine-rich repeat-containing subgroup of the G protein-coupled 7-transmembrane protein superfamily. Lgr6 is a high affinity receptor for R-Spondins 1-3 and potentially functions as a tumor suppressor despite its positive effect on Wnt/ beta -Catenin signaling.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 6

Entrez Gene IDs

59352 (Human); 329252 (Mouse); 498233 (Rat)

Alternate Names

FLJ14471; gonadotropin receptor; GPCR; leucine-rich repeat containing G protein-coupled receptor 6; leucine-rich repeat-containing G protein-coupled receptor 6; leucine-rich repeat-containing G-protein coupled receptor 6; Lgr6; VTS20631