Human LIF Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cell Proliferation Induced by LIF and Neutralization by Human LIF Antibody. Recombinant Human LIF stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by 1.5 ng/mL Recombinant Human LIF is neutralized (green line) by increasing concentrations of Mouse Anti-Human LIF Monoclonal Antibody (Catalog # MAB250). The ND50 is typically 0.06-0.2 µg/mL.
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Detection of Human LIF by Western Blot Autocrine IL-6 activates STAT3 signalling in lung cancer cell-induced epidural ADSCs. a Epidural ADSCs were pre-treated with CM from lung cancer cells for 48 h, and pSTAT3 and STAT3 expression levels were detected by western blotting. Epidural ADSCs cultured in untreated medium served as a control. b The effects of lung cancer cell CM on epidural ADSC proliferation were evaluated using the CCK-8 assay. Epidural ADSCs were treated with CM from one of four lung cancer cell lines, and the optical density of both groups at 450 nm was analysed. Data from three separate experiments are shown. c Western blot analysis of pSTAT3 and STAT3 in epidural ADSCs treated with either 10 ng/mL recombinant IL-6, 10 ng/mL recombinant IL-11 or 50 ng/mL recombinant LIF in the presence or absence of either neutralizing antibodies or isotype controls. Loading control, actin. d Western blot analysis of pSTAT3 and alpha -SMA expression in epidural ADSCs treated with lung cancer cell CM in the presence or absence of neutralizing antibodies against IL-6, IL-11 or LIF. Loading control, actin. *P < 0.05; **P < 0.01; ***P < 0.001 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31196220), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human LIF by Western Blot Autocrine IL-6 activates STAT3 signalling in lung cancer cell-induced epidural ADSCs. a Epidural ADSCs were pre-treated with CM from lung cancer cells for 48 h, and pSTAT3 and STAT3 expression levels were detected by western blotting. Epidural ADSCs cultured in untreated medium served as a control. b The effects of lung cancer cell CM on epidural ADSC proliferation were evaluated using the CCK-8 assay. Epidural ADSCs were treated with CM from one of four lung cancer cell lines, and the optical density of both groups at 450 nm was analysed. Data from three separate experiments are shown. c Western blot analysis of pSTAT3 and STAT3 in epidural ADSCs treated with either 10 ng/mL recombinant IL-6, 10 ng/mL recombinant IL-11 or 50 ng/mL recombinant LIF in the presence or absence of either neutralizing antibodies or isotype controls. Loading control, actin. d Western blot analysis of pSTAT3 and alpha -SMA expression in epidural ADSCs treated with lung cancer cell CM in the presence or absence of neutralizing antibodies against IL-6, IL-11 or LIF. Loading control, actin. *P < 0.05; **P < 0.01; ***P < 0.001 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31196220), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LIF
LIF is a 36‑67 kDa highly glycosylated polypeptide (1, 2) produced by a variety of cells including T cells (3), monocytes (4), fibroblasts (5), osteoblasts (6) and mast cells (7). Consistent with its many synonyms, LIF exhibits a broad spectrum of effects on both hematopoietic and nonhematopoietic cells. For example, LIF inhibits the differentiation of embryonic stem cells (8), up regulates the synthesis of acute phase proteins in hepatocytes (9), down regulates lipoprotein lipase activity in adipocytes (10), and preferentially induces a cholinergic phenotype in sympathetic neurons (11). The receptor for LIF (LIF R) has been isolated and found to be a 190 kDa type I transmembrane glycoprotein (12). Although this molecule binds LIF, the resultant LIF-LIF R complex is not sufficient to transduce an intracellular signal. This capability is provided by a 130 kDa signal transducing subunit (gp130) that is common to the functional receptors for IL-6, IL-11, CNTF, and Oncostatin M (13, 14). Since gp130 is a ubiquitously expressed membrane protein, the presence of LIF R (membrane-bound or soluble form) ultimately determines the cell’s responsiveness to LIF. Cells known to express LIF R include osteoblasts (6), hepatocytes (15), macrophages (15), neurons (5), and megakaryocytes (16). Human and mouse LIF exhibit 78% sequence homology, and human LIF is biologically active on mouse cells (17).
- Van-Vlasselaev, P. et al. (1992) Prog. Growth Factor Res. 4:337.
- Gough, N.M. (1992) Growth Factors 7:175.
- Anegon, N.M, I. et al. (1991) J. Immunol.147:3973.
- Gillett, N.A. et al. (1993) Growth Factors 9:301.
- Banner, L. R. and P.H. Patterson (1994) Proc. Natl. Acad. Sci. 91:7109.
- Allen, E.H. et al. (1990) J. Cell. Physiol. 145:110.
- Lorenzo, J.A. et al. (1994) Clin.Immunol. Immunopathol. 70:260.
- Williams, R.L. et al. (1988) Nature 336:684.
- Baumann, H. et al. (1989) J. Immunol. 143:1163.
- Marshall, M.K. et al. (1994) Endocrinology 135:1412.
- Ludham, W.H. et al. (1994) Dev. Biol. 164:5283.
- Davis, S. et al. (1993) Science 260:18054.
- Gearing, D.P. et al. (1991) EMBO J. 10:28395.
- Gearing, D.P. et al. (1991) Science 255:1434.
- Hilton, D.J. and N.A. Nicola (1992) J. Biol. Chem. 267:102286.
- Hilton, D.J. et al. (1992) Ciba17. Foundation Symposium 167:2278.
Product Datasheets
Citations for Human LIF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Downregulation of miR-211-5p Promotes Carboplatin Resistance in Human Retinoblastoma Y79 Cells by Affecting the GDNF–LIF Interaction
Authors: Ning Ke, Lin Chen, Qing Liu, Haibo Xiong, Xinke Chen, Xiyuan Zhou
Frontiers in Oncology
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Protein Kinase Inhibitor gamma Reciprocally Regulates Osteoblast and Adipocyte Differentiation by Downregulating Leukemia Inhibitory Factor
Authors: Xin Chen, Bryan S. Hausman, Guangbin Luo, Guang Zhou, Shunichi Murakami, Janet Rubin et al.
Stem Cells
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Microarray and Proteomic Analysis of Breast Cancer Cell and Osteoblast Co-cultures: ROLE OF OSTEOBLAST MATRIX METALLOPROTEINASE (MMP)-13 IN BONE METASTASIS.
Authors: Morrison C, Mancini S, Cipollone J, Kappelhoff R, Roskelley C, Overall C
J. Biol. Chem., 2011-07-22;286(39):34271-85.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Maternal Leukemia Inhibitory Factor (LIF) Promotes Fetal Neurogenesis via a LIF-ACTH-LIF Signaling Relay Pathway
Authors: Eriko Simamura, Hiroki Shimada, Nobuaki Higashi, Maimi Uchishiba, Hiroki Otani, Toshihisa Hatta
Endocrinology
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Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
Blood, 2007-09-11;110(13):4319-30.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Neutralization -
Immunocytochemical detection and RT-PCR expression of leukaemia inhibitory factor and its receptor in human fetal and adult ovaries.
Authors: Abir R, Fisch B, Jin S, Barnnet M, Freimann S, van den Hurk R, Feldberg D, Nitke S, Krissi H, Ao A
Mol. Hum. Reprod., 2004-03-25;10(5):313-9.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
The reprogrammed host: Chlamydia trachomatis-induced up-regulation of glycoprotein 130 cytokines, transcription factors, and antiapoptotic genes.
Authors: Hess S, 2019, Rheinheimer C, Tidow F, Bartling G, Kaps C, Lauber J, Buer J, Klos A
7046, 2001-10-01;44(10):2392-401.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Therapeutic benefits of factors derived from stem cells from human exfoliated deciduous teeth for radiation-induced mouse xerostomia
Authors: F Kano, N Hashimoto, Y Liu, L Xia, T Nishihara, W Oki, K Kawarabaya, N Mizusawa, K Aota, T Sakai, M Azuma, H Hibi, T Iwasaki, T Iwamoto, N Horimai, A Yamamoto
Scientific Reports, 2023-02-15;13(1):2706.
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