Human LIGHT/TNFSF14 APC-conjugated Antibody
Human LIGHT/TNFSF14 APC-conjugated Antibody Summary
Asp74-Val240
Accession # O43557
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of LIGHT/TNFSF14 in PBMC treated with PMA and Calcium Ionomycin vs resting PBMC by Flow Cytometry PBMC treated with 50ng/mL PMA and 500ng/mL Calcium Ionomycin for 48 hours (A) vs resting PBMC (B) were stained with Mouse Anti-Human LIGHT/TNFSF14 APC‑conjugated Monoclonal Antibody (Catalog # FAB664A) and Mouse Anti-Human CD8 alpha PE‑conjugated Monoclonal Antibody (Catalog # FAB1509P) isotype control antibody (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LIGHT/TNFSF14
Human LIGHT, also known as TNFSF14, is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to Lymphotoxins, exhibits Inducible expression, and competes with HSV Glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the Lymphotoxin beta Receptor (LT beta R) and the decoy receptor, DcR3/TR6. LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma.
- Mauri, D.N. et al. (1998) Immunity 8:21.
- Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
- Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
- Yu, K-Y. et al. (1999) J. Biol. Chem. 274:13733.
Product Datasheets
Citation for Human LIGHT/TNFSF14 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Licensed human natural killer cells aid dendritic cell maturation via TNFSF14/LIGHT.
Authors: Holmes T, Wilson E, Black E, Benest A, Vaz C, Tan B, Tanavde V, Cook G
Proc Natl Acad Sci U S A, 2014-12-15;111(52):E5688-96.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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